Stefano Del Duca Dipartimento di Scienze Biologiche, Geologiche ed Ambientali, University of Bologna, Italy
1 protocol

Giampiero Cai
  • Dipartimento Scienze della Vita, University of Siena, Italy
  • 1 Author merit


Ph.D. in Environmental Biology, University of Siena, Italy, 1993

Current Position

Assistant Professor, University of Siena, Italy
Research interests: Growth of the pollen tube; Cytoskeleton dynamics and functions in plants; Expression of tubulin isoforms; Deposition and assembly of the cell wall; Environmental stress and plant cells

Publications (selected)

  1. Parrotta, L., Cresti, M. and Cai, G. (2013). Heat-shock protein 70 binds microtubules and interacts with kinesin in tobacco pollen tubes. Cytoskeleton (Hoboken) 70(9): 522-537.
  2. Del Duca, S., Serafini-Fracassini, D. and Cai, G. (2013). An unconventional road for the secretion of transglutaminase in pollen tubes? Plant Signal Behav 8(6).
  3. Del Duca, S., Faleri, C., Iorio, R. A., Cresti, M., Serafini-Fracassini, D. and Cai, G. (2013). Distribution of transglutaminase in pear pollen tubes in relation to cytoskeleton and membrane dynamics. Plant Physiol 161(4): 1706-1721.
  4. Nepi, M., Soligo, C., Nocentini, D., Abate, M., Guarnieri, M., Cai, G., Bini, L., Puglia, M., Bianchi, L. and Pacini, E. (2012). Amino acids and protein profile in floral nectar: much more than a simple reward. Flora-Morphology, Distribution, Functional Ecology of Plants.
  5. Nicolardi, V., Cai, G., Parrotta, L., Puglia, M., Bianchi, L., Bini, L. and Gaggi, C. (2012). The adaptive response of lichens to mercury exposure involves changes in the photosynthetic machinery. Environ Pollut 160(1): 1-10.
  6. Cai, G., Faleri, C., Cresti, M., Di Sandro, A., Serafini-Fracassini, D. and Del Duca, S. (2011). Pollen Tube Cytoskeleton, Transglutaminases and Self-Incompatibility. Acta Horticulturae 967. 149-156.
  7. Aloisi, I., Del Duca, S., De Franceschi, P., Dondini, L., Iorio, R., Serafini-Fracassini, D., Sansavini, S., Cai, G. and Faleri, C. (2011). Post-Translational Modification by Transglutaminase of Proteins Involved in Pear Self-Incompatibility. Acta Horticulturae 967. 141-148.
  8. Nepi, M., Bini, L., Bianchi, L., Puglia, M., Abate, M. and Cai, G. (2011). Xylan-degrading enzymes in male and female flower nectar of Cucurbita pepo. Ann Bot 108(3): 521-527.
  9. Cai, G. (2011). How do microtubules affect deposition of cell wall polysaccharides in the pollen tube? Plant Signal Behav 6(5): 732-735.
  10. Cai, G., Faleri, C., Del Casino, C., Emons, A. M. and Cresti, M. (2011). Distribution of callose synthase, cellulose synthase, and sucrose synthase in tobacco pollen tube is controlled in dissimilar ways by actin filaments and microtubules. Plant Physiol 155(3): 1169-1190.
  11. Cai, G. and Cresti, M. (2010). Microtubule motors and pollen tube growth--still an open question. Protoplasma 247(3-4): 131-143.
  12. Di Sandro, A., Del Duca, S., Verderio, E., Hargreaves, A. J., Scarpellini, A., Cai, G., Cresti, M., Faleri, C., Iorio, R. A., Hirose, S., Furutani, Y., Coutts, I. G., Griffin, M., Bonner, P. L. and Serafini-Fracassini, D. (2010). An extracellular transglutaminase is required for apple pollen tube growth. Biochem J 429(2): 261-271.
  13. Cai, G. (2010). Assembly and disassembly of plant microtubules: tubulin modifications and binding to MAPs. J Exp Bot 61(3): 623-626.
  14. Del Duca, S., Cai, G., Di Sandro, A. and Serafini-Fracassini, D. (2010). Compatible and self-incompatible pollination in Pyrus communis displays different polyamine levels and transglutaminase activity. Amino Acids 38(2): 659-667.
  15. Parrotta, L., Cai, G. and Cresti, M. (2010). Changes in the accumulation of α- and β-tubulin during bud development in Vitis vinifera L. Planta 231(2): 277-291.
  16. Del Duca, S., Serafini-Fracassini, D., Bonner, P., Cresti, M. and Cai, G. (2009). Effects of post-translational modifications catalysed by pollen transglutaminase on the functional properties of microtubules and actin filaments. Biochem J 418(3): 651-664.
  17. Cai, G. and Cresti, M. (2009). Organelle motility in the pollen tube: a tale of 20 years. J Exp Bot 60(2): 495-508.
  18. Di Sandro, A., Serafini-Fracassini, D., Del Duca, S., Della Mea, M., Faleri, C., Cai, G., De Franceschi, P., Dondini, L. and Sansavini, S. (2007). Pollen Transglutaminase in Pear Self Incompatibility and Relationships with S-RNases and S-Allele Variability. Acta Horticulturae, 800(1), 423-429.
  19. Persia, D., Cai, G., Del Casino, C., Faleri, C., Willemse, M. T. and Cresti, M. (2008). Sucrose synthase is associated with the cell wall of tobacco pollen tubes. Plant Physiol 147(4): 1603-1618.
  20. Romagnoli, S., Cai, G., Faleri, C., Yokota, E., Shimmen, T. and Cresti, M. (2007). Microtubule- and actin filament-dependent motors are distributed on pollen tube mitochondria and contribute differently to their movement. Plant Cell Physiol 48(2): 345-361.
  21. Cai, G. and Cresti, M. (2006). The microtubular cytoskeleton in pollen tubes: structure and role in organelle trafficking. The Pollen Tube, Springer: 157-175.
  22. Cai, G., Del Casino, C., Romagnoli, S. and Cresti, M. (2005). Pollen cytoskeleton during germination and tube growth. CURRENT SCIENCE-BANGALORE- 89(11): 1853.
  23. Cai, G., Ovidi, E., Romagnoli, S., Vantard, M., Cresti, M. and Tiezzi, A. (2005). Identification and characterization of plasma membrane proteins that bind to microtubules in pollen tubes and generative cells of tobacco. Plant Cell Physiol 46(4): 563-578.
  24. Romagnoli, S., Cai, G. and Cresti, M. (2003). Kinesin-like proteins and transport of pollen tube organelles. Cell Biol Int 27(3): 255-256.
  25. Romagnoli, S., Cai, G. and Cresti, M. (2003). In vitro assays demonstrate that pollen tube organelles use kinesin-related motor proteins to move along microtubules. Plant Cell 15(1): 251-269.
1 Protocol published
Binding Assay of Cytosolic Proteins to the Cytoskeleton
Authors:  Stefano Del Duca and Giampiero Cai, date: 11/20/2013, view: 4891, Q&A: 0
Many cellular proteins interact with the cytoskeleton (both actin filaments and microtubules), either dynamically or permanently. This interaction is required during different aspects of the cell life, for example during the process of cell ...