Malika Khakimova Department of Microbiology, McGill University, Canada
2 protocols

Dao Nguyen
  • Department of Medicine, McGill University, Canada
Research focus
  • Microbiology
  • 2 Author merit


M.Sc. in Epidemiology and Biostatistics, McGill University, 2004

M.D.CM, Faculty of Medicine, McGill University, Montreal, 1997

Current position

Assistant professor, Department of Medicine, Faculty of Medicine, McGill University

Associate member, Departments of Microbiology and Immunology, and Experimental Medicine

Full-time clinical staff, Division of Respirology, McGill University Health Center


  1. Beaudoin, T., LaFayette, S., Roussel, L., Bérubé, J., Desrosiers, M., Nguyen, D. and Rousseau, S. (2013). The level of p38α MAPK activation in airway epithelial cells determines the onset of innate immune responses to planktonic and biofilm Pseudomonas aeruginosa. Journal of Infectious Diseases.
  2. Khakimova, M., Ahlgren, H. G., Harrison, J. J., English, A. M. and Nguyen, D. (2013). The Stringent Response Controls Catalases in Pseudomonas aeruginosa and Is Required for Hydrogen Peroxide and Antibiotic Tolerance. J Bacteriol 195(9): 2011-2020.
  3. Beaudoin, T., LaFayette, S., Nguyen, D. and Rousseau, S. (2012). Mucoid Pseudomonas aeruginosa caused by mucA mutations result in activation of TLR2 in addition to TLR5 in airway epithelial cells. Biochem Biophys Res Commun. 428(1):150-4.
  4. Nguyen, D.*, Joshi-Datar, A., Lepine, F., Bauerle, E., Olakanmi, O., Beer, K., McKay, G., Siehnel, R., Schafhauser, J. and Wang, Y. (2011). Active starvation responses mediate antibiotic tolerance in biofilms and nutrient-limited bacteria. Science 334(6058): 982-986. *Corresponding author
    Recommended in Faculty of 1000 as a “must read” publication.
    Featured in Scientific American Health News
    Highlighted in Nature Microbiology reviews
  5. Pepperell, C. S., Granka, J. M., Alexander, D. C., Behr, M. A., Chui, L., Gordon, J., Guthrie, J. L., Jamieson, F. B., Langlois-Klassen, D., Long, R., Nguyen, D., Wobeser, W. and Feldman, M. W. (2011). Dispersal of Mycobacterium tuberculosis via the Canadian fur trade. Proc Natl Acad Sci U S A 108(16): 6526-6531.
  6. Nguyen, D., Emond, M. J., Mayer‐Hamblett, N., Saiman, L., Marshall, B. C. and Burns, J. L. (2007). Clinical response to azithromycin in cystic fibrosis correlates with in vitro effects on Pseudomonas aeruginosa phenotypes. Pediatric pulmonology 42(6): 533-541.    
  7. Nguyen, D. and Singh, P. K. (2006). Evolving stealth: genetic adaptation of Pseudomonas aeruginosa during cystic fibrosis infections. Proceedings of the National Academy of Sciences 103(22): 8305-8306.
2 Protocols published
H2O2 Kill Assays of Planktonic Stationary Phase Bacteria
Authors:  Malika Khakimova and Dao Nguyen, date: 11/05/2013, view: 8745, Q&A: 0
Stationary phase bacteria are highly tolerant to hydrogen peroxide. This protocol was developed to test the susceptibility to hydrogen peroxide killing in different Pseudomonas aeruginosa strains. This assay provides a reliable way to ...
H2O2 Kill Assays of Biofilm Bacteria
Authors:  Malika Khakimova and Dao Nguyen, date: 11/05/2013, view: 9615, Q&A: 0
Ubiquitous in nature and often surface associated, biofilms cause numerous chronic human infections. Biofilms are structured multicellular bacterial communities where cells are entrapped in a polymer matrix. Bacteria growing as biofilms are ...
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