Hilde Houtte VIB Department of Molecular Microbiology, KU Leuven Laboratory of Molecular Cell Biology, Belgium
1 protocol

Patrick Van Dijck
  • VIB Department of Molecular Microbiology, KU Leuven Laboratory of Molecular Cell Biology, Belgium
Research focus
  • Plant science
  • 1 Author merit


Ph.D. at the laboratory for Experimental Medicine and Endocrinology (LEGENDO) at Gasthuisberg, KU Leuven, Belgium, 1991

Current position

Full professor (Gewoon Hoogleraar) at KU Leuven, Group leader VIB

Publications (since 2008)

Publications in International journals

  1. De Graeve S., Marinelli, S., Stolz, F., Hendrickx, J., Vandamme, J., Engelborghs, Y., Van Dijck, P. and Thevelein, J. M. Mammalian ribosomal and chaperone protein RPS3A counteracts alpha-Synuclein aggregation and toxicity in a yeast model system Biochemical Journal In press
  2. Alsteens, D., Van Dijck, P., Lipke, P. N. and Dufrêne, Y. F. Quantifying the forces driving cell-cell adhesion in a fungal pathogen. Langmuir Submitted
  3. Beaussart, A., Herman, P., El-Kirat-Chatel, S., Lipke, P., Kucharikova, S., Van Dijck, P. and Dufrêne, Y. Single-cell force spectroscopy of the medically-important Staphylococcus epidermidis-Candida albicans interaction. Nanoscale In press
  4. Tournu, H., Fiori, A., Van Dijck, P. Relevance of trehalose in pathogenicity: some general rules, yet many exceptions. PLoS Pathogens In press
  5. Mathé, L. and Van Dijck, P. Recent insights into Candida albicans biofilm resistance mechanisms. Current Genetics In press
  6. Vande Velde, G., Kucharikova, S., Schrevens, S., Himmelreich, U., Van Dijck, P. Towards non-invasive monitoring of pathogen-host interactions during Candida albicans biofilm formation using in vivo bioluminescence. Cellular Microbiology In press
  7. Vande Velde, G., Kucharikova ,S., Van Dijck, P., Himmelreich, U. Bioluminescence imaging of fungal biofilm development in live animals. Methods in Molecular Biology In press
  8. Rosa, J. C., Colombo, L. T., Alvim, M. C., Avonce, N., Van Dijck, P. and Passos, F. M. (2013). Metabolic engineering of Kluyveromyces lactis for L-ascorbic acid (vitamin C) biosynthesis. Microb Cell Fact 12(1): 59.
  9. Van Houtte, H., Vandesteene, L., Lopez-Galvis, L., Lemmens, L., Kissel, E., Carpentier, S., Feil, R., Avonce, N., Beeckman, T., Lunn, J. E. and Van Dijck, P. (2013). Overexpression of the trehalase gene AtTRE1 leads to increased drought stress tolerance in Arabidopsis and is involved in abscisic acid-induced stomatal closure. Plant Physiol 161(3): 1158-1171.
  10. Van Houtte, H., Lopez-Galvis, L., Vandesteene, L., Beeckman, T. and Van Dijck, P. (2013). Redundant and non-redundant roles of the trehalose-6-phosphate phosphatases in leaf growth, root hair specification and energy-responses in Arabidopsis. Plant Signal Behav 8(3).
  11. Kucharikova, S., Sharma, N., Spriet, I., Maertens, J., Van Dijck, P. and Lagrou, K. (2013). Activities of systemically administered echinocandins against in vivo mature Candida albicans biofilms developed in a rat subcutaneous model. Antimicrob Agents Chemother 57(5): 2365-2368.
  12. Beaussart, A., Alsteens, D., El-Kirat-Chatel, S., Lipke, P. N., Kucharikova, S., Van Dijck, P. and Dufrene, Y. F. (2012). Single-molecule imaging and functional analysis of Als adhesins and mannans during Candida albicans morphogenesis. ACS Nano 6(12): 10950-10964.
  13. Bink, A., Kucharikova, S., Neirinck, B., Vleugels, J., Van Dijck, P., Cammue, B. P. and Thevissen, K. (2012). The nonsteroidal antiinflammatory drug diclofenac potentiates the in vivo activity of caspofungin against Candida albicans biofilms. J Infect Dis 206(11): 1790-1797.
  14. Fiori, A., Kucharikova, S., Govaert, G., Cammue, B. P., Thevissen, K. and Van Dijck, P. (2012). The heat-induced molecular disaggregase Hsp104 of Candida albicans plays a role in biofilm formation and pathogenicity in a worm infection model. Eukaryot Cell 11(8): 1012-1020.
  15. Serneels, J., Tournu, H. and Van Dijck, P. (2012). Tight control of trehalose content is required for efficient heat-induced cell elongation in Candida albicans. J Biol Chem 287(44): 36873-36882.
  16. Zimon, M., Baets, J., Almeida-Souza, L., De Vriendt, E., Nikodinovic, J., Parman, Y., Battaloglu, E., Matur, Z., Guergueltcheva, V., Tournev, I., Auer-Grumbach, M., De Rijk, P., Petersen, B. S., Muller, T., Fransen, E., Van Damme, P., Loscher, W. N., Barisic, N., Mitrovic, Z., Previtali, S. C., Topaloglu, H., Bernert, G., Beleza-Meireles, A., Todorovic, S., Savic-Pavicevic, D., Ishpekova, B., Lechner, S., Peeters, K., Ooms, T., Hahn, A. F., Zuchner, S., Timmerman, V., Van Dijck, P., Rasic, V. M., Janecke, A. R., De Jonghe, P. and Jordanova, A. (2012). Loss-of-function mutations in HINT1 cause axonal neuropathy with neuromyotonia. Nat Genet 44(10): 1080-1083.
  17. Bink, A., Govaert, G., Vandenbosch, D., Kucharikova, S., Coenye, T., Nelis, H., Van Dijck, P., Cammue, B. P. and Thevissen, K. (2012). Transcription factor Efg1 contributes to the tolerance of Candida albicans biofilms against antifungal agents in vitro and in vivo. J Med Microbiol 61(Pt 6): 813-819.
  18. Vandesteene, L., Lopez-Galvis, L., Vanneste, K., Feil, R., Maere, S., Lammens, W., Rolland, F., Lunn, J. E., Avonce, N., Beeckman, T. and Van Dijck, P. (2012). Expansive evolution of the trehalose-6-phosphate phosphatase gene family in Arabidopsis. Plant Physiol 160(2): 884-896.
  19. Fiori, A. and Van Dijck, P. (2012). Potent synergistic effect of doxycycline with fluconazole against Candida albicans is mediated by interference with iron homeostasis. Antimicrob Agents Chemother 56(7): 3785-3796.
  20. Stynen, B., Tournu, H., Tavernier, J. and Van Dijck, P. (2012). Diversity in genetic in vivo methods for protein-protein interaction studies: from the yeast two-hybrid system to the mammalian split-luciferase system. Microbiol Mol Biol Rev 76(2): 331-382.
  21. Van Houdt, J. K., Nowakowska, B. A., Sousa, S. B., van Schaik, B. D., Seuntjens, E., Avonce, N., Sifrim, A., Abdul-Rahman, O. A., van den Boogaard, M. J., Bottani, A., Castori, M., Cormier-Daire, V., Deardorff, M. A., Filges, I., Fryer, A., Fryns, J. P., Gana, S., Garavelli, L., Gillessen-Kaesbach, G., Hall, B. D., Horn, D., Huylebroeck, D., Klapecki, J., Krajewska-Walasek, M., Kuechler, A., Lines, M. A., Maas, S., Macdermot, K. D., McKee, S., Magee, A., de Man, S. A., Moreau, Y., Morice-Picard, F., Obersztyn, E., Pilch, J., Rosser, E., Shannon, N., Stolte-Dijkstra, I., Van Dijck, P., Vilain, C., Vogels, A., Wakeling, E., Wieczorek, D., Wilson, L., Zuffardi, O., van Kampen, A. H., Devriendt, K., Hennekam, R. and Vermeesch, J. R. (2012). Heterozygous missense mutations in SMARCA2 cause Nicolaides-Baraitser syndrome. Nat Genet 44(4): 445-449, S441.
  22. Tournu, H. and Van Dijck, P. (2012). Candida biofilms and the host: models and new concepts for eradication. Int J Microbiol 2012: 845352.
  23. Paul, M. and Van Dijck, P. (2011). How do sugars regulate plant growth? Front Plant Sci 2: 90.
  24. Kraidlova, L., Van Zeebroeck, G., Van Dijck, P. and Sychrova, H. (2011). The Candida albicans GAP gene family encodes permeases involved in general and specific amino acid uptake and sensing. Eukaryot Cell 10(9): 1219-1229.
  25. Kucharikova, S., Tournu, H., Lagrou, K., Van Dijck, P. and Bujdakova, H. (2011). Detailed comparison of Candida albicans and Candida glabrata biofilms under different conditions and their susceptibility to caspofungin and anidulafungin. J Med Microbiol 60(Pt 9): 1261-1269.
  26. Rotthier, A., Auer-Grumbach, M., Janssens, K., Baets, J., Penno, A., Almeida-Souza, L., Van Hoof, K., Jacobs, A., De Vriendt, E., Schlotter-Weigel, B., Loscher, W., Vondracek, P., Seeman, P., De Jonghe, P., Van Dijck, P., Jordanova, A., Hornemann, T. and Timmerman, V. (2010). Mutations in the SPTLC2 subunit of serine palmitoyltransferase cause hereditary sensory and autonomic neuropathy type I. Am J Hum Genet 87(4): 513-522.
  27. Kucharikova, S., Tournu, H., Holtappels, M., Van Dijck, P. and Lagrou, K. (2010). In vivo efficacy of anidulafungin against mature Candida albicans biofilms in a novel rat model of catheter-associated Candidiasis. Antimicrob Agents Chemother 54(10): 4474-4475.
  28. Vianna, C. R., Ferreira, M. C., Silva, C. L., Tanghe, A., Neves, M. J., Thevelein, J. M., Rosa, C. A. and Van Dijck, P. (2010). Stress tolerance of the Saccharomyces cerevisiae adenylate cyclase fil1 (CYR1) mutant depends on Hsp26. J Mol Microbiol Biotechnol 19(3): 140-146.
  29. Stynen, B., Van Dijck, P. and Tournu, H. (2010). A CUG codon adapted two-hybrid system for the pathogenic fungus Candida albicans. Nucleic Acids Res 38(19): e184.
  30. Wilson, D., Fiori, A., Brucker, K. D., Dijck, P. V. and Stateva, L. (2010). Candida albicans Pde1p and Gpa2p comprise a regulatory module mediating agonist-induced cAMP signalling and environmental adaptation. Fungal Genet Biol 47(9): 742-752.
  31. Vandesteene, L., Ramon, M., Le Roy, K., Van Dijck, P. and Rolland, F. (2010). A single active trehalose-6-P synthase (TPS) and a family of putative regulatory TPS-like proteins in Arabidopsis. Mol Plant 3(2): 406-419.
  32. Nailis, H., Kucharikova, S., Ricicova, M., Van Dijck, P., Deforce, D., Nelis, H. and Coenye, T. (2010). Real-time PCR expression profiling of genes encoding potential virulence factors in Candida albicans biofilms: identification of model-dependent and -independent gene expression. BMC Microbiol 10: 114.
  33. Kuchariková, S., Van Dijck, P., Lisalová, M. and Bujdaková, H. (2010). Efficiency of azoles, amphotericin B and echinocandins on itraconazole resistant Candida glabrata clinical isolates. Central European Journal of Biology 5, 318-323.
  34. Ricicova, M., Kucharikova, S., Tournu, H., Hendrix, J., Bujdakova, H., Van Eldere, J., Lagrou, K. and Van Dijck, P. (2010). Candida albicans biofilm formation in a new in vivo rat model. Microbiology 156(Pt 3): 909-919.
  35. Avonce, N., Wuyts, J., Verschooten, K., Vandesteene, L. and Van Dijck, P. (2010). The Cytophaga hutchinsonii ChTPSP: First characterized bifunctional TPS-TPP protein as putative ancestor of all eukaryotic trehalose biosynthesis proteins. Mol Biol Evol 27(2): 359-369.
  36. Anthonio, E. A., Brees, C., Baumgart-Vogt, E., Hongu, T., Huybrechts, S. J., Van Dijck, P., Mannaerts, G. P., Kanaho, Y., Van Veldhoven, P. P. and Fransen, M. (2009). Small G proteins in peroxisome biogenesis: the potential involvement of ADP-ribosylation factor 6. BMC Cell Biol 10: 58.
  37. Storkebaum, E., Leitao-Goncalves, R., Godenschwege, T., Nangle, L., Mejia, M., Bosmans, I., Ooms, T., Jacobs, A., Van Dijck, P., Yang, X. L., Schimmel, P., Norga, K., Timmerman, V., Callaerts, P. and Jordanova, A. (2009). Dominant mutations in the tyrosyl-tRNA synthetase gene recapitulate in Drosophila features of human Charcot-Marie-Tooth neuropathy. Proc Natl Acad Sci U S A 106(28): 11782-11787.
  38. Ramon, M., De Smet, I., Vandesteene, L., Naudts, M., Leyman, B., Van Dijck, P., Rolland, F., Beeckman, T. and Thevelein, J. M. (2009). Extensive expression regulation and lack of heterologous enzymatic activity of the Class II trehalose metabolism proteins from Arabidopsis thaliana. Plant Cell Environ 32(8): 1015-1032.
  39. Van Dijck, P. (2009). Nutrient sensing G protein-coupled receptors: interesting targets for antifungals? Med Mycol 47(7): 671-680.
  40. Van Laere, S. D., Verstrepen, K. J., Thevelein, J. M., Vandijck, P. and Delvaux, F. R. (2008). Formation of higher alcohols and their acetate esters. CEREVISIA 33(2): 65-81.
  41. Van Laere, S. D., Saerens, S. M., Verstrepen, K. J., Van Dijck, P., Thevelein, J. M. and Delvaux, F. R. (2008). Flavour formation in fungi: characterisation of KlAtf, the Kluyveromyces lactis orthologue of the Saccharomyces cerevisiae alcohol acetyltransferases Atf1 and Atf2. Appl Microbiol Biotechnol 78(5): 783-792.
  42. Stasyk, O. G., Maidan, M. M., Stasyk, O. V., Van Dijck, P., Thevelein, J. M. and Sibirny, A. A. (2008). Identification of hexose transporter-like sensor HXS1 and functional hexose transporter HXT1 in the methylotrophic yeast Hansenula polymorpha. Eukaryot Cell 7(4): 735-746.
  43. Maere, S., Van Dijck, P. and Kuiper, M. (2008). Extracting expression modules from perturbational gene expression compendia. BMC Syst Biol 2: 33.
  44. Maidan, M. M., De Rop, L., Relloso, M., Diez-Orejas, R., Thevelein, J. M. and Van Dijck, P. (2008). Combined inactivation of the Candida albicans GPR1 and TPS2 genes results in avirulence in a mouse model for systemic infection. Infect Immun 76(4): 1686-1694.
  45. Saerens, S. M., Delvaux, F., Verstrepen, K. J., Van Dijck, P., Thevelein, J. M. and Delvaux, F. R. (2008). Parameters affecting ethyl ester production by Saccharomyces cerevisiae during fermentation. Appl Environ Microbiol 74(2): 454-461.

Articles in books/book chapters

  1. Stynen, B., Van Dijck, P. and Tournu, H. (2012). Cool Tools 1: Development and application of a Candida albicans two-hybrid system In Candida and Candidiasis, 2nd Edition. R.A. Calderone and C.J. Clancy (eds). ASM press.
  2. Walker, G. and Van Dijck P. (2006). Physiological and molecular responses of yeasts to the environment. In Yeast Handbook vol 2: Yeasts in Food and Beverages:. Chapter 5 A. Querol and G. Fleet (eds). Springer Verlag, pp 111-152.
  3. Tanghe, A., Van Dijck, P. and Thevelein, J.M. (2005). Determinants of freeze tolerance in micro-organisms, physiological importance and biotechnological applications. In: Adv. Appl. Microbiol. AI Laskin, JW Bennet and GM Gadd. (eds). Elsevier Academic Press, 53:129-176.
  4. Leyman B., N. Avonce, M. Ramon, P. Van Dijck, J.M. (2004).Thevelein and G. Iturriaga New selection marker for plant transformation. In: 'Recombinant Gene Expression. Reviews and Protocols', 2nd edition. Paulina Balbas and Argelia Lorence (eds). Series: Methods in Molecular Biology. J. Walker (eds). Humana Press pp.385-396.
1 Protocol published
Trehalase Activity in Arabidopsis thaliana Optimized for 96-well Plates
Authors:  Hilde Van Houtte and Patrick Van Dijck, date: 10/20/2013, view: 10423, Q&A: 2
Trehalose is a nonreducing disaccharide. It is a common sugar in bacteria, fungi and yeast, where it functions as a carbon source and stress protectant. In contrast, plants, although encoding large trehalose biosynthesis gene families, contain only ...
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