Education

Ph.D. in Biological Sciences, Cambridge University,1990

Current position

Associate Professor in Molecular Microbiology, School of Life Sciences, University of Nottingha, UK

Publications (since 2005)

Peer reviewed articles

1. Randle, J., Metcalfe, J., Webb, H., Luckett, JCA, Nerlich, B., Vaughan, N. Segal, J., and Hardie, K.R. (2013). Impact of an educational intervention upon the hand hygiene compliance of children. Journal of Hospital Infection. In press (IF 3.221)
   
2. Baban, S.T., Kuehne, S. A., Barketi-Klai, A., Cartman, S.T., Kelly, M., Hardie, K. R., Kansau, I., Collignon, A., Minton, N.P. (2013). The role of flagella in Clostridium difficile pathogenesis: Comparison between a non-epidemic and an epidemic strain. Plos One In press (IF 3.73)
   
3. Luckett, J. C., Darch, O., Watters, C., Abuoun, M., Wright, V., Paredes-Osses, E., Ward, J., Goto, H., Heeb, S., Pommier, S., Rumbaugh, K. P., Camara, M. and Hardie, K. R. (2012). A novel virulence strategy for Pseudomonas aeruginosa mediated by an autotransporter with arginine-specific aminopeptidase activity. PLoS Pathog 8(8): e1002854.
   
4. Hardie, K.R. (2011). To spin-out or to license? That is the question. Microbiology Today (SGM) August 41:168-69.
   
5. Ortori, C. A., Dubern, J. F., Chhabra, S. R., Camara, M., Hardie, K., Williams, P. and Barrett, D. A. (2011). Simultaneous quantitative profiling of N-acyl-L-homoserine lactone and 2-alkyl-4(1H)-quinolone families of quorum-sensing signaling molecules using LC-MS/MS. Anal Bioanal Chem 399(2): 839-850
   
6. Hardie, K.R. and Verran, J. (2010). Come Dine with Microbes. Microbiology Today. August 37:190-191.
   
7. Shen, F., Hobley, L., Doherty, N., Loh, J. T., Cover, T. L., Sockett, R. E., Hardie, K. R. and Atherton, J. C. (2010). In Helicobacter pylori auto-inducer-2, but not LuxS/MccAB catalysed reverse transsulphuration, regulates motility through modulation of flagellar gene transcription. BMC Microbiol 10: 210.
   
8. Halliday, N. M., Hardie, K. R., Williams, P., Winzer, K. and Barrett, D. A. (2010). Quantitative liquid chromatography-tandem mass spectrometry profiling of activated methyl cycle metabolites involved in LuxS-dependent quorum sensing in Escherichia coli. Anal Biochem 403(1-2): 20-29.
   
9. Doherty, N. C., Shen, F., Halliday, N. M., Barrett, D. A., Hardie, K. R., Winzer, K. and Atherton, J. C. (2010). In Helicobacter pylori, LuxS is a key enzyme in cysteine provision through a reverse transsulfuration pathway. J Bacteriol 192(5): 1184-1192.
   
10. O'Toole, P. W., Snelling, W. J., Canchaya, C., Forde, B. M., Hardie, K. R., Josenhans, C., Graham, R., McMullan, G., Parkhill, J., Belda, E. and Bentley, S. D. (2010). Comparative genomics and proteomics of Helicobacter mustelae, an ulcerogenic and carcinogenic gastric pathogen. BMC Genomics 11: 164.
    
11. Holmes, K., Tavender, T. J., Winzer, K., Wells, J. M. and Hardie, K. R. (2009). AI-2 does not function as a quorum sensing molecule in Campylobacter jejuni during exponential growth in vitro. BMC Microbiol 9: 214.
    
12. Heurlier, K., Vendeville, A., Halliday, N., Green, A., Winzer, K., Tang, C. M. and Hardie, K. R. (2009). Growth deficiencies of Neisseria meningitidis pfs and luxS mutants are not due to inactivation of quorum sensing. J Bacteriol 191(4): 1293-1302.
    
13. Tavender, T. J., Halliday, N. M., Hardie, K. R. and Winzer, K. (2008). LuxS-independent formation of AI-2 from ribulose-5-phosphate. BMC Microbiol 8: 98.
    
14. Argent, R. H., Thomas, R. J., Letley, D. P., Rittig, M. G., Hardie, K. R. and Atherton, J. C. (2008). Functional association between the Helicobacter pylori virulence factors VacA and CagA. J Med Microbiol 57(Pt 2): 145-150.
    
15. Wand, M. E., Sockett, R. E., Evans, K. J., Doherty, N., Sharp, P. M., Hardie, K. R. and Winzer, K. (2006). Helicobacter pylori FlhB function: the FlhB C-terminal homologue HP1575 acts as a "spare part" to permit flagellar export when the HP0770 FlhBCC domain is deleted. J Bacteriol 188(21): 7531-7541.
    
16. Fitchen, N., Letley, D. P., O'Shea, P., Atherton, J. C., Williams, P. and Hardie, K. R. (2005). All subtypes of the cytotoxin VacA adsorb to the surface of Helicobacter pylori post-secretion. J Med Microbiol 54(Pt 7): 621-630.
    

Reviews

1. Hardie, K. R. and Heurlier, K. (2008). Establishing bacterial communities by 'word of mouth': LuxS and autoinducer 2 in biofilm development. Nat Rev Microbiol 6(8): 635-643.
   
2. Vendeville, A., Winzer, K., Heurlier, K., Tang, C. M. and Hardie, K. R. (2005). Making 'sense' of metabolism: autoinducer-2, LuxS and pathogenic bacteria. Nat Rev Microbiol 3(5): 383-396.
   
3. Fitchen, N., O'Shea, P., Williams, P. and Hardie, K. R. (2003). Electrostatic sensor for identifying interactions between peptides and bacterial membranes. Mol Immunol 40(7): 407-411.
   
4. Winzer, K., Hardie, K. R. and Williams, P. (2002). Bacterial cell-to-cell communication: sorry, can't talk now - gone to lunch! Curr Opin Microbiol 5(2): 216-222.
   
5. Pugsley, A., Francetic, O., Hardie, K., Possot, O., Sauvonnet, N. and Seydel, A. (1997). Pullulanase: model protein substrate for the general secretory pathway of gram-negative bacteria. Folia microbiologica 42(3): 184-192.
   
6. Pugsley, A. P., Francetic, O., Possot, O. M., Sauvonnet, N. and Hardie, K. R. (1997). Recent progress and future directions in studies of the main terminal branch of the general secretory pathway in Gram-negative bacteria--a review. Gene 192(1): 13-19.
   
7. Hughes, C., Hardie, K.R., Koronakis, E., Bailey, M., Stanley, P. and Koronakis, V. (1994). Synthesis, maturation and secretion of E. coli hemolysin and related toxins. Proceedings of the Sixth European Toxin Symposium: In Zeitallblatt fur Bakteriologie. Fisher Verlog. In Bacterial protein toxins p183.
   
8. Hughes, C., Issartel, J-P., Hardie, K.R., Stanley, P., Koronakis, E., and Koronakis, V. (1992). Activation of E. coli prohemolysin to the membrane-targeted toxin by HlyC-directed ACP-dependent fatty acylation. FEMS Microbiology Immunology 105:37-44.
   

Books

1. Hardie, K.R., Pommier, S., and Wilhelm, S. (2009). The secreted proteins of Pseudomonas aeruginosa: their export machineries, and how they contribute to pathogenesis. Bacterial secreted proteins and pathogenesis Horizon Bioscience ISBN: 978-1-904455-42
   
2. Hardie, K.R, Baldwin, T., and Williams, P. (2005). Molecular basis of bacterial adaptation to a pathogenic lifestyle. Topley and Wilson’s Microbiology and Microbial Infections: Bacteriology. 10^th Edition. Hodder Arnold Volume 1 chapter 6: 146-183.
   
3. Winzer, K., Hardie, K.R., and Williams, P. (2003). LuxS and autoinducer-2: their contribution to quorum sensing and metabolism in bacteria. Advances in Applied Microbiology, Academic Press, San Diego, USA. Volume 53: 291-396.
   
4. Hardie, K.R. and Williams, P. (1998). Fractionation of bacterial cell envelopes. Methods in Microbiology: Bacterial Pathogenesis, Academic Press. Eds: Paul Williams, Peter Williams, George Salmond, Julian Ketley.
   
5. Filloux, A. and Hardie, K. R. (1998). A systematic approach to the study of protein secretion in Gram-negative bacteria. Methods in Microbiology:Bacterial Pathogenesis, Academic Press. Eds: Paul Williams, Peter Williams, George Salmond, Julian Ketley.