Kim Hardie School of Life Sciences, University of Nottingham, United Kingdom
2 protocols
Alexander Westbye NIOZ Royal Netherlands Institute for Sea Research
6 protocols

Amit Dey International Centre for Genetic Engineering and Biotechnology
14 protocols

Anna Zorina Russian Academy of Sciences
5 protocols

David Paul MRC Laboratory of Molecular Biology
35 protocols

Reviewer
Esteban Paredes-Osses
  • Research scientist, Institute of Public Health
Research focus
  • Microbiology
  • 1 Author merit

Education

PhD, University of Nottigham, 2015

Publications

https://www.ncbi.nlm.nih.gov/pubmed/28283946

https://www.ncbi.nlm.nih.gov/pubmed/22927813
1 Protocol published
Cell Fractionation of Pseudomonas aeruginosa
Authors:  Esteban Paredes-Osses and Kim R. Hardie, date: 10/05/2013, view: 9667, Q&A: 1
Pseudomonas aeruginosa is a Gram negative bacterium. Separating the cell envelope compartments enables proteins to be localized to confirm where in the cell they function. Cell fractionation can also provide a first step in a protein ...
7 Protocols reviewed
Microtubule Seeded-assembly in the Presence of Poorly Nucleating Nucleotide Analogues
Authors:  Siou Ku, Claire Heichette, Laurence Duchesne and Denis Chrétien, date: 08/20/2020, view: 574, Q&A: 0
Microtubule dynamic instability is driven by the hydrolysis of the GTP bound to the β-subunit of the α-β tubulin heterodimer. Nucleotide analogues are commonly used to mimic the different steps of the tubulin GTPase cycle, but most of them are poor ...
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F-actin Bundle Sedimentation Assay
Authors:  Shan-Shan Lin, Mei-Chun Chuang and Ya-Wen Liu, date: 11/05/2019, view: 2025, Q&A: 0
Understanding the molecular mechanism governing the higher-order regulation of actin dynamics requires chemically-defined and quantitative assays. Recently, the membrane remodeling large GTPase, dynamin, has been identified as a new actin ...
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