Josse Galen Cell and Developmental Biology Unit, Centre Regulacio Genomica, Spain
1 protocol

Julia von Blume
  • Molecular Medicine Division, Max Planck Institute of Biochemistry, Germany
  • 1 Author merit
1 Protocol published
Enrichment of Golgi membranes from HeLa cells by sucrose gradient ultracentrifugation
Authors:  Josse van Galen and Julia von Blume, date: 09/20/2013, view: 8006, Q&A: 1
This is a protocol to extract intact Golgi Membranes from HeLa cells using sucrose gradient centrifugation. This extraction is very useful for several applications including pull-down of Golgi membrane proteins, electron microscopy and ...