Timo Lehti University of Helsinki
1 protocol

Benita Westerlund-Wikström
  • Department of Biosciences, University of Helsinki, Finland
  • 1 Author merit

Education

Ph.D., University of Helsinki, 1991

Current position

University lecturer, Department of Biosciences, University of Helsinki, Finland

Fields of research: Molecular microbiology, microbe-host interactions, bacterial protein secretion, bacterial surface display

Publications (since 2005)

  1. Lehti, T. A., Bauchart, P., Kukkonen, M., Dobrindt, U., Korhonen, T. K. and Westerlund-Wikstrom, B. (2013). Phylogenetic group-associated differences in regulation of the common colonization factor Mat fimbria in Escherichia coli. Mol Microbiol 87(6): 1200-1222.
  2. Korhonen, T. K., Haiko, J., Laakkonen, L., Jarvinen, H. M. and Westerlund-Wikstrom, B. (2013). Fibrinolytic and coagulative activities of Yersinia pestis. Front Cell Infect Microbiol 3: 35.
  3. Lehti, T. A., Bauchart, P., Dobrindt, U., Korhonen, T. K. and Westerlund-Wikstrom, B. (2012). The fimbriae activator MatA switches off motility in Escherichia coli by repression of the flagellar master operon flhDC. Microbiology 158(Pt 6): 1444-1455.
  4. Lehti, T. A., Heikkinen, J., Korhonen, T. K. and Westerlund-Wikstrom, B. (2012). The response regulator RcsB activates expression of Mat fimbriae in meningitic Escherichia coli. J Bacteriol 194(13): 3475-3485.
  5. Edelman, S. M., Lehti, T. A., Kainulainen, V., Antikainen, J., Kylvaja, R., Baumann, M., Westerlund-Wikstrom, B. and Korhonen, T. K. (2012). Identification of a high-molecular-mass Lactobacillus epithelium adhesin (LEA) of Lactobacillus crispatus ST1 that binds to stratified squamous epithelium. Microbiology 158(Pt 7): 1713-1722.
  6. Kylvaja, R., Kankainen, M., Holm, L. and Westerlund-Wikstrom, B. (2011). Adhesive polypeptides of Staphylococcus aureus identified using a novel secretion library technique in Escherichia coli. BMC Microbiol 11: 117.
  7. Haiko, J., Laakkonen, L., Westerlund-Wikstrom, B. and Korhonen, T. K. (2011). Molecular adaptation of a plant-bacterium outer membrane protease towards plague virulence factor Pla. BMC Evol Biol 11: 43.
  8. Kylli, P., Nohynek, L., Puupponen-Pimia, R., Westerlund-Wikstrom, B., Leppanen, T., Welling, J., Moilanen, E. and Heinonen, M. (2011). Lingonberry (Vaccinium vitis-idaea) and European cranberry (Vaccinium microcarpon) proanthocyanidins: isolation, identification, and bioactivities. J Agric Food Chem 59(7): 3373-3384.
  9. Kylli, P., Nohynek, L., Puupponen-Pimia, R., Westerlund-Wikstrom, B., McDougall, G., Stewart, D. and Heinonen, M. (2010). Rowanberry phenolics: compositional analysis and bioactivities. J Agric Food Chem 58(22): 11985-11992.
  10. Lehti, T. A., Bauchart, P., Heikkinen, J., Hacker, J., Korhonen, T. K., Dobrindt, U. and Westerlund-Wikstrom, B. (2010). Mat fimbriae promote biofilm formation by meningitis-associated Escherichia coli. Microbiology 156(Pt 8): 2408-2417.
  11. Anton, L., Majander, K., Savilahti, H., Laakkonen, L. and Westerlund-Wikstrom, B. (2010). Two distinct regions in the model protein Peb1 are critical for its heterologous transport out of Escherichia coli. Microb Cell Fact 9: 97.
  12. Ojala, T., Kuparinen, V., Koskinen, J. P., Alatalo, E., Holm, L., Auvinen, P., Edelman, S., Westerlund-Wikstrom, B., Korhonen, T. K., Paulin, L. and Kankainen, M. (2010). Genome sequence of lactobacillus crispatus ST1. J. Bacteriol. 192: 3547-3548.
  13. Sjostrom, A. E., Balsalobre, C., Emody, L., Westerlund-Wikstrom, B., Hacker, J. and Uhlin, B. E. (2009). The SfaXII protein from newborn meningitis E. coli is involved in regulation of motility and type 1 fimbriae expression. Microb Pathog 46(5): 243-252.
  14. Haiko, J., Kukkonen, M., Ravantti, J. J., Westerlund-Wikstrom, B. and Korhonen, T. K. (2009). The single substitution I259T, conserved in the plasminogen activator Pla of pandemic Yersinia pestis branches, enhances fibrinolytic activity. J Bacteriol 191(15): 4758-4766.
  15. Majander, K., Anton, L., Kylvaja, R. and Westerlund-Wikstr?m, B. (2009). The bacterial flagellum as a surface display and expression tool. Pili and flagella: Current research and future trends (ed. K. Jarrell): 191-206.
  16. Suomalainen, M., Haiko, J., Ramu, P., Lobo, L., Kukkonen, M., Westerlund-Wikstrom, B., Virkola, R., Lahteenmaki, K. and Korhonen, T. K. (2007). Using every trick in the book: the Pla surface protease of Yersinia pestis. Adv Exp Med Biol 603: 268-278.
  17. Majander, K., Korhonen, T. K. and Westerlund-Wikstrom, B. (2005). Simultaneous display of multiple foreign peptides in the FliD capping and FliC filament proteins of the Escherichia coli flagellum. Appl Environ Microbiol 71(8): 4263-4268.
  18. Majander, K., Anton, L., Antikainen, J., Lang, H., Brummer, M., Korhonen, T. K. and Westerlund-Wikstrom, B. (2005). Extracellular secretion of polypeptides using a modified Escherichia coli flagellar secretion apparatus. Nat Biotechnol 23(4): 475-481.
  19. Westerlund-Wikstrom, B. and Korhonen, T. K. (2005). Molecular structure of adhesin domains in Escherichia coli fimbriae. Int J Med Microbiol 295(6-7): 479-486.
1 Protocol published
Colony Immunoblotting Assay for Detection of Bacterial Cell-surface or Extracellular Proteins
Authors:  Timo A. Lehti and Benita Westerlund-Wikström, date: 09/05/2013, view: 17225, Q&A: 1
This simple protocol describes how to detect antigens from agar-grown bacterial colonies transferred to nitrocellulose using specific antibodies. The protocol is well suitable for detection of bacterial proteins exposed on the cell surface or ...
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