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Yukio Nagano Analytical Research Center for Experimental Sciences, Saga University, Japan
1 protocol

Kenta Goto
  • Analytical Research Center for Experimental Sciences, Saga University, Japan
  • 1 Author merit
1 Protocol published
Ultra-low Background DNA Cloning System
Authors:  Yukio Nagano and Kenta Goto, date: 08/20/2013, view: 8925, Q&A: 0
We have developed a method to clone DNA fragments into the E. coli plasmid vectors with almost 100% efficiency (Goto and Nagano, 2013). This method is based on highly efficient yeast-based in vivo cloning, and the subsequent ...