Dear Drs. Huei San Leong and Marnie Blewitt,
Hello!
After transduction the prepared Retrovirus Mediated Malignant Transformation of MEF to mouse, in wild type created neoplasm and dramatic tumor grow, however, it could not create in a gene Knockout mouse. What is the possibility, or reason and how to resolve the problem?
If shRNA-p53 transfection is a must step in the oncogenic c-myc puro seleated Myc MEFs or oncogenic Ras puro selected Ras MEFs?
Thanks for your comment and help!
9/13/2014 8:56:43 AM Reply