Amy Palin Department of Pediatrics and Program in Immunology, Stanford University Medical School, USA
1 protocol

David B. Lewis
  • Department of Pediatrics, Stanford University Medical School, USA
Research focus
  • Immunology
  • 1 Author merit


M.D., University of California, San Francisco, CA, 1981

Current position

Professor of Pediatrics, Stanford University, Stanford, CA

Chief of Division of Immunology, Allergy, and Rheumatology, Stanford, CA

Publications (since 2005)

  1. Palin, A. C., Ramachandran, V., Acharya, S. and Lewis, D. B. (2013). Human neonatal naive CD4+ T cells have enhanced activation-dependent signaling regulated by the microRNA miR-181a. J Immunol 190(6): 2682-2691.
  2. Bhaumik, S., Giffon, T., Bolinger, D., Kirkman, R., Lewis, D. B., Weaver, C. T. and Randolph, D. A. (2013). Retinoic acid hypersensitivity promotes peripheral tolerance in recent thymic emigrants. J Immunol 190(6): 2603-2613.
  3. Zheng, J., Liu, Y., Liu, M., Xiang, Z., Lam, K. T., Lewis, D. B., Lau, Y. L. and Tu, W. (2013). Human CD8+ regulatory T cells inhibit GVHD and preserve general immunity in humanized mice. Sci Transl Med 5(168): 168ra169.
  4. Baradaran-Heravi, A., Cho, K. S., Tolhuis, B., Sanyal, M., Morozova, O., Morimoto, M., Elizondo, L. I., Bridgewater, D., Lubieniecka, J., Beirnes, K., Myung, C., Leung, D., Fam, H. K., Choi, K., Huang, Y., Dionis, K. Y., Zonana, J., Keller, K., Stenzel, P., Mayfield, C., Lucke, T., Bokenkamp, A., Marra, M. A., van Lohuizen, M., Lewis, D. B., Shaw, C. and Boerkoel, C. F. (2012). Penetrance of biallelic SMARCAL1 mutations is associated with environmental and genetic disturbances of gene expression. Hum Mol Genet 21(11): 2572-2587.
  5. Dong, L., Liu, F., Fairman, J., Hong, D. K., Lewis, D. B., Monath, T., Warner, J. F., Belser, J. A., Patel, J., Hancock, K., Katz, J. M. and Lu, X. (2012). Cationic liposome-DNA complexes (CLDC) adjuvant enhances the immunogenicity and cross-protective efficacy of a pre-pandemic influenza A H5N1 vaccine in mice. Vaccine 30(2): 254-264.
  6. Lewis, D. B., Haines, C. and Ross, D. (2011). Protein tyrosine kinase 7: a novel surface marker for human recent thymic emigrants with potential clinical utility. J Perinatol 31 Suppl 1: S72-81.
  7. Yu, G. P., Nadeau, K. C., Berk, D. R., de Saint Basile, G., Lambert, N., Knapnougel, P., Roberts, J., Kavanau, K., Dunn, E., Stiehm, E. R., Lewis, D. B., Umetsu, D. T., Puck, J. M. and Cowan, M. J. (2011). Genotype, phenotype, and outcomes of nine patients with T-B+NK+ SCID. Pediatr Transplant 15(7): 733-741.
  8. Zheng, J., Liu, Y., Qin, G., Lam, K. T., Guan, J., Xiang, Z., Lewis, D. B., Lau, Y. L. and Tu, W. (2011). Generation of human Th1-like regulatory CD4+ T cells by an intrinsic IFN-gamma- and T-bet-dependent pathway. Eur J Immunol 41(1): 128-139.
  9. Hong, D. K., Tremoulet, A. H., Burns, J. C. and Lewis, D. B. (2011). Cross-reactive neutralizing antibody against pandemic 2009 H1N1 influenza a virus in intravenous immunoglobulin preparations. Pediatr Infect Dis J 30(1): 67-69.
  10. Weinstock, D., Lewis, D. B., Parker, G. A., Beyer, J., Collinge, M., Brown, T. P. and Dybdal, N. (2010). Toxicopathology of the developing immune system: investigative and development strategies. Toxicol Pathol 38(7): 1111-1117.
  11. Hong, D. K., Chang, S., Botham, C. M., Giffon, T. D., Fairman, J. and Lewis, D. B. (2010). Cationic lipid/DNA complex-adjuvanted influenza A virus vaccination induces robust cross-protective immunity. J Virol 84(24): 12691-12702.
  12. Nelson, K. S. and Lewis, D. B. (2010). Adult-onset presentations of genetic immunodeficiencies: genes can throw slow curves. Curr Opin Infect Dis 23(4): 359-364.
  13. O'Gorman, W. E., Sampath, P., Simonds, E. F., Sikorski, R., O'Malley, M., Krutzik, P. O., Chen, H., Panchanathan, V., Chaudhri, G., Karupiah, G., Lewis, D. B., Thorne, S. H. and Nolan, G. P. (2010). Alternate mechanisms of initial pattern recognition drive differential immune responses to related poxviruses. Cell Host Microbe 8(2): 174-185.
  14. Zheng, J., Liu, Y., Qin, G., Chan, P. L., Mao, H., Lam, K. T., Lewis, D. B., Lau, Y. L. and Tu, W. (2009). Efficient induction and expansion of human alloantigen-specific CD8 regulatory T cells from naive precursors by CD40-activated B cells. J Immunol 183(6): 3742-3750.
  15. Matute, J. D., Arias, A. A., Wright, N. A., Wrobel, I., Waterhouse, C. C., Li, X. J., Marchal, C. C., Stull, N. D., Lewis, D. B., Steele, M., Kellner, J. D., Yu, W., Meroueh, S. O., Nauseef, W. M. and Dinauer, M. C. (2009). A new genetic subgroup of chronic granulomatous disease with autosomal recessive mutations in p40 phox and selective defects in neutrophil NADPH oxidase activity. Blood 114(15): 3309-3315.
  16. Lay, M., Callejo, B., Chang, S., Hong, D. K., Lewis, D. B., Carroll, T. D., Matzinger, S., Fritts, L., Miller, C. J., Warner, J. F., Liang, L. and Fairman, J. (2009). Cationic lipid/DNA complexes (JVRS-100) combined with influenza vaccine (Fluzone) increases antibody response, cellular immunity, and antigenically drifted protection. Vaccine 27(29): 3811-3820.
  17. Haines, C. J., Giffon, T. D., Lu, L. S., Lu, X., Tessier-Lavigne, M., Ross, D. T. and Lewis, D. B. (2009). Human CD4+ T cell recent thymic emigrants are identified by protein tyrosine kinase 7 and have reduced immune function. J Exp Med 206(2): 275-285.
  18. Tu, W., Lau, Y. L., Zheng, J., Liu, Y., Chan, P. L., Mao, H., Dionis, K., Schneider, P. and Lewis, D. B. (2008). Efficient generation of human alloantigen-specific CD4+ regulatory T cells from naive precursors by CD40-activated B cells. Blood 112(6): 2554-2562.
  19. Dvorak, C. C., Sandford, A., Fong, A., Cowan, M. J., George, T. I. and Lewis, D. B. (2008). Maternal T-cell engraftment associated with severe hemophagocytosis of the bone marrow in untreated X-linked severe combined immunodeficiency. J Pediatr Hematol Oncol 30(5): 396-400.
  20. Zeman, A. M., Holmes, T. H., Stamatis, S., Tu, W., He, X. S., Bouvier, N., Kemble, G., Greenberg, H. B., Lewis, D. B., Arvin, A. M. and Dekker, C. L. (2007). Humoral and cellular immune responses in children given annual immunization with trivalent inactivated influenza vaccine. Pediatr Infect Dis J 26(2): 107-115.
  21. He, X. S., Holmes, T. H., Zhang, C., Mahmood, K., Kemble, G. W., Lewis, D. B., Dekker, C. L., Greenberg, H. B. and Arvin, A. M. (2006). Cellular immune responses in children and adults receiving inactivated or live attenuated influenza vaccines. J Virol 80(23): 11756-11766.
  22. Tu, W., Potena, L., Stepick-Biek, P., Liu, L., Dionis, K. Y., Luikart, H., Fearon, W. F., Holmes, T. H., Chin, C., Cooke, J. P., Valantine, H. A., Mocarski, E. S. and Lewis, D. B. (2006). T-cell immunity to subclinical cytomegalovirus infection reduces cardiac allograft disease. Circulation 114(15): 1608-1615.
  23. Cohen, A. C., Nadeau, K. C., Tu, W., Hwa, V., Dionis, K., Bezrodnik, L., Teper, A., Gaillard, M., Heinrich, J., Krensky, A. M., Rosenfeld, R. G. and Lewis, D. B. (2006). Cutting edge: Decreased accumulation and regulatory function of CD4+ CD25(high) T cells in human STAT5b deficiency. J Immunol 177(5): 2770-2774.
  24. Potena, L., Holweg, C. T., Chin, C., Luikart, H., Weisshaar, D., Narasimhan, B., Fearon, W. F., Lewis, D. B., Cooke, J. P., Mocarski, E. S. and Valantine, H. A. (2006). Acute rejection and cardiac allograft vascular disease is reduced by suppression of subclinical cytomegalovirus infection. Transplantation 82(3): 398-405.
  25. Chen, L., Cohen, A. C. and Lewis, D. B. (2006). Impaired allogeneic activation and T-helper 1 differentiation of human cord blood naive CD4 T cells. Biol Blood Marrow Transplant 12(2): 160-171.
  26. Zhou, B., Comeau, M. R., De Smedt, T., Liggitt, H. D., Dahl, M. E., Lewis, D. B., Gyarmati, D., Aye, T., Campbell, D. J. and Ziegler, S. F. (2005). Thymic stromal lymphopoietin as a key initiator of allergic airway inflammation in mice. Nat Immunol 6(10): 1047-1053.
1 Protocol published
Transfection of Human Naive CD4+ T Cells with PHA Activation and Neon Electroporation
Authors:  Amy Palin and David B. Lewis, date: 08/05/2013, view: 13653, Q&A: 0
Transfection of primary T cells can be challenging. This protocol describes a method to transfect primary human naive CD4+ T cells with an AP-1 luciferase reporter using low-level activation by phytohemagglutinin (PHA) and ...
We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.