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Coauthors
Antimo Migliaccio Department of Biochemistry, Biophysics and General Pathology, Second University of Naples, Italy
1 protocol

Gabriella Castoria Department of Biochemistry, Biophysics and General Pathology, Second University of Naples, Italy
1 protocol
Coreviewers
Alessandro Didonna University of California, San Francisco
14 protocols

Emma Puighermanal Universitat Autònoma de Barcelona
2 protocols

Gal Haimovich Molecular Genetics, Weizmann Institute of Science, Israel
42 protocols

Hsin-Yi Chang Kyoto University
7 protocols

Reviewer
Pia Giovannelli
  • Post-Doc, Second University of Naples
Research focus
  • Cancer biology
  • Cancer, prostate, androgen receptor, steroid hormones, breast cancer, estradiol receptor
  • 1 Author merit
1 Protocol published
Estradiol Receptor (ER) Chromatin Immunoprecipitation in MCF-7 Cells
Authors:  Pia Giovannelli, Gabriella Castoria and Antimo Migliaccio, date: 07/20/2013, view: 5302, Q&A: 0
Steroid hormone receptors, for example estradiol receptor, act like transcription factors. In the cell, steroids bind to a specific receptor. Upon ligand binding, many steroid receptors dimerize and enter nuclei where they bind specific DNA ...
15 Protocols reviewed
Fluorophore-Based Mitochondrial Ca2+ Uptake Assay
Author:  Charles B. Phillips, date: 07/20/2018, view: 244, Q&A: 0
The physiological importance of mitochondrial calcium uptake, observed in processes such as ATP production, intracellular calcium signaling, and apoptosis, makes desirable a simple, straightforward way of investigating this event with unambiguous ...
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γ-Secretase Epsilon-cleavage Assay
γ-Secretase epsilon-cleavage assay is derived from the cell-based Tango assay (Kang et al., 2015), and is a fast and sensitive method to determine the initial cleavage of C99 by γ-secretase. In this protocol, we use HTL cells, which are ...
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