Pia Giovannelli —BIO-PROTOCOL

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Coauthors

Antimo Migliaccio Department of Biochemistry, Biophysics and General Pathology, Second University of Naples, Italy
1 protocol

Gabriella Castoria Department of Biochemistry, Biophysics and General Pathology, Second University of Naples, Italy
1 protocol

Coreviewers

Alessandro Didonna University of California, San Francisco
12 protocols

Emma Puighermanal Universitat Autònoma de Barcelona
2 protocols

Gal Haimovich Molecular Genetics, Weizmann Institute of Science, Israel
40 protocols

Hsin-Yi Chang Kyoto University
7 protocols

Reviewer

Pia Giovannelli

Post-Doc, Second University of Naples

Research focus

Cancer biology
Cancer, prostate, androgen receptor, steroid hormones, breast cancer, estradiol receptor

1 Author merit
Reviewer merit
Editor merit

Education

PhD, 2010

Lab information

Antimo Migliaccio Laboratory
http://www.dbbpg.unina2.it/attachments/article/96/Castoria.pdf

Publications

https://www.ncbi.nlm.nih.gov/pubmed/?term=Giovannelli+P

1 Protocol published

Estradiol Receptor (ER) Chromatin Immunoprecipitation in MCF-7 Cells

Authors: Pia Giovannelli, Gabriella Castoria and Antimo Migliaccio, date: 07/20/2013, view: 5024, Q&A: 0

Steroid hormone receptors, for example estradiol receptor, act like transcription factors. In the cell, steroids bind to a specific receptor. Upon ligand binding, many steroid receptors dimerize and enter nuclei where they bind specific DNA ...

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14 Protocols reviewed

γ-Secretase Epsilon-cleavage Assay

Authors: Ting-Hai Xu, Yan Yan, Kaleeckal G. Harikumar, Laurence J. Miller, Karsten Melcher and H. Eric Xu, date: 11/20/2017, view: 1375, Q&A: 0

γ-Secretase epsilon-cleavage assay is derived from the cell-based Tango assay (Kang et al., 2015), and is a fast and sensitive method to determine the initial cleavage of C99 by γ-secretase. In this protocol, we use HTL cells, which are ...

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The Long-lived Protein Degradation Assay: an Efficient Method for Quantitative Determination of the Autophagic Flux of Endogenous Proteins in Adherent Cell Lines

Authors: Morten Luhr, Frank Sætre and Nikolai Engedal, date: 05/05/2018, view: 781, Q&A: 0

Autophagy is a key player in the maintenance of cellular homeostasis in eukaryotes, and numerous diseases, including cancer and neurodegenerative disorders, are associated with alterations in autophagy. The interest for studying autophagy has grown ...

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