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Coauthors
Antimo Migliaccio Department of Biochemistry, Biophysics and General Pathology, Second University of Naples, Italy
1 protocol

Gabriella Castoria Department of Biochemistry, Biophysics and General Pathology, Second University of Naples, Italy
1 protocol
Coreviewers
Emma Puighermanal Université Montpellier
1 protocol

Hsin-Yi Chang Kyoto University
7 protocols

Jyotiska Chaudhuri Buck Institute for Research on Aging
26 protocols

Kate Hannan The Australian National University
11 protocols

Reviewer
Pia Giovannelli
  • Post-Doc, Second University of Naples
Research focus
  • Cancer biology
  • Cancer, prostate, androgen receptor, steroid hormones, breast cancer, estradiol receptor
  • 1 Author merit

Education

PhD, 2010

Lab information

Antimo Migliaccio Laboratory
http://www.dbbpg.unina2.it/attachments/article/96/Castoria.pdf

Publications

https://www.ncbi.nlm.nih.gov/pubmed/?term=Giovannelli+P

1 Protocol published
Estradiol Receptor (ER) Chromatin Immunoprecipitation in MCF-7 Cells
Authors:  Pia Giovannelli, Gabriella Castoria and Antimo Migliaccio, date: 07/20/2013, view: 4541, Q&A: 0
Steroid hormone receptors, for example estradiol receptor, act like transcription factors. In the cell, steroids bind to a specific receptor. Upon ligand binding, many steroid receptors dimerize and enter nuclei where they bind specific DNA ...
13 Protocols reviewed
γ-Secretase Epsilon-cleavage Assay
γ-Secretase epsilon-cleavage assay is derived from the cell-based Tango assay (Kang et al., 2015), and is a fast and sensitive method to determine the initial cleavage of C99 by γ-secretase. In this protocol, we use HTL cells, which are ...
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A General Method for Intracellular Protein Delivery through ‘E-tag’ Protein Engineering and Arginine Functionalized Gold Nanoparticles
Authors:  Rubul Mout and Vincent M. Rotello, date: 12/20/2017, view: 638, Q&A: 0
In this protocol, we describe a method for direct cytosolic protein delivery that avoids endosomal entrapment of the delivered proteins. We achieved this by tagging the desired protein with an oligo glutamic acid tag (E-tag), and subsequently using ...
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