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Antimo Migliaccio Department of Biochemistry, Biophysics and General Pathology, Second University of Naples, Italy
 1 protocol

Gabriella Castoria Department of Biochemistry, Biophysics and General Pathology, Second University of Naples, Italy
 1 protocol
Alessandro Didonna University of California, San Francisco
 16 protocols

Emma Puighermanal Universitat Autònoma de Barcelona
 2 protocols

Gal Haimovich Molecular Genetics, Weizmann Institute of Science, Israel
 47 protocols

Hsin-Yi Chang Kyoto University
 7 protocols

Reviewer
Pia Giovannelli
  • Post-Doc, Second University of Naples
Research focus
  • Cancer biology
  • Cancer, prostate, androgen receptor, steroid hormones, breast cancer, estradiol receptor
  • 1 Author merit
1 Protocol published
Estradiol Receptor (ER) Chromatin Immunoprecipitation in MCF-7 Cells
Authors:  Pia Giovannelli, Gabriella Castoria and Antimo Migliaccio, date: 07/20/2013, view: 5877, Q&A: 0
Steroid hormone receptors, for example estradiol receptor, act like transcription factors. In the cell, steroids bind to a specific receptor. Upon ligand binding, many steroid receptors dimerize and enter nuclei where they bind specific DNA ...
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15 Protocols reviewed
Fluorophore-Based Mitochondrial Ca2+ Uptake Assay
Author:  Charles B. Phillips, date: 07/20/2018, view: 725, Q&A: 0
The physiological importance of mitochondrial calcium uptake, observed in processes such as ATP production, intracellular calcium signaling, and apoptosis, makes desirable a simple, straightforward way of investigating this event with unambiguous ...
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γ-Secretase Epsilon-cleavage Assay
Authors:  Ting-Hai Xu, Yan Yan, Kaleeckal G. Harikumar, Laurence J. Miller, Karsten Melcher and H. Eric Xu, date: 11/20/2017, view: 2217, Q&A: 0
γ-Secretase epsilon-cleavage assay is derived from the cell-based Tango assay (Kang et al., 2015), and is a fast and sensitive method to determine the initial cleavage of C99 by γ-secretase. In this protocol, we use HTL cells, which are ...
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