Christian Schwarz Molecular Plant Science, Ludwig-Maximilians-Universität, Germany
2 protocols

Alexandra Bohne Ludwig Maximilians University
1 protocol

Joerg Nickelsen
  • Molecular Plant Science, Ludwig-Maximilians-Universität, Germany
Research focus
  • Plant science
  • 2 Author merit


Ph.D., Plant Molecular Biology, Ruhr University Bochum, Germany, 1992

Current position

Professor in Molecular Plant Science at the Ludwig-Maximilians-University Munich

Publications (since 2005)

  1. Rengstl, B., Knoppova, J., Komenda, J. and Nickelsen, J. (2013). Characterization of a Synechocystis double mutant lacking the photosystem II assembly factors YCF48 and Sll0933. Planta 237(2): 471-480.
  2. Nickelsen, J. and Rengstl, B. (2013). Photosystem II Assembly: From Cyanobacteria to Plants. Annu Rev Plant Biol 64: 609-635.
  3. Bohne, A. V., Schwarz, C., Schottkowski, M., Lidschreiber, M., Piotrowski, M., Zerges, W. and Nickelsen, J. (2013). Reciprocal regulation of protein synthesis and carbon metabolism for thylakoid membrane biogenesis. PLoS Biol 11(2): e1001482.
  4. Komenda, J., Knoppova, J., Kopecna, J., Sobotka, R., Halada, P., Yu, J., Nickelsen, J., Boehm, M. and Nixon, P. J. (2012). The Psb27 assembly factor binds to the CP43 complex of photosystem II in the cyanobacterium Synechocystis sp. PCC 6803. Plant Physiol 158(1): 476-486.
  5. Nickelsen, J. and Bohne, A.V. (2012) Plastid Transformation in Algae. In: Bock, R. and Knoop, V. (eds) Genomics of Chloroplasts and Mitochondria. Adv. in Photosyn. and Resp. 35, 379-392.
  6. Stengel, A., Gugel, I. L., Hilger, D., Rengstl, B., Jung, H. and Nickelsen, J. (2012). Initial steps of photosystem II de novo assembly and preloading with manganese take place in biogenesis centers in Synechocystis. Plant Cell 24(2): 660-675.
  7. Schwarz, C., Bohne, A. V., Wang, F., Cejudo, F. J. and Nickelsen, J. (2012). An intermolecular disulfide-based light switch for chloroplast psbD gene expression in Chlamydomonas reinhardtii. Plant J 72(3): 378-389.
  8. Rengstl, B., Stengel, A., Gügel, I., Schottkowski, M., and Nickelsen, J. (2012) Biogenesis of thylakoid membranes in Synechocystis sp. PCC 6803. Endocyt. Cell Res., 23, 86-90.
  9. Nickelsen, J., Rengstl, B., Stengel, A., Schottkowski, M., Soll, J. and Ankele, E. (2011). Biogenesis of the cyanobacterial thylakoid membrane system--an update. FEMS Microbiol Lett 315(1): 1-5.
  10. Rengstl, B., Oster, U., Stengel, A. and Nickelsen, J. (2011). An intermediate membrane subfraction in cyanobacteria is involved in an assembly network for Photosystem II biogenesis. J Biol Chem 286(24): 21944-21951.
  11. Johnson, X., Wostrikoff, K., Finazzi, G., Kuras, R., Schwarz, C., Bujaldon, S., Nickelsen, J., Stern, D. B., Wollman, F. A. and Vallon, O. (2010). MRL1, a conserved Pentatricopeptide repeat protein, is required for stabilization of rbcL mRNA in Chlamydomonas and Arabidopsis. Plant Cell 22(1): 234-248.
  12. Dorn, K. V., Willmund, F., Schwarz, C., Henselmann, C., Pohl, T., Hess, B., Veyel, D., Usadel, B., Friedrich, T., Nickelsen, J. and Schroda, M. (2010). Chloroplast DnaJ-like proteins 3 and 4 (CDJ3/4) from Chlamydomonas reinhardtii contain redox-active Fe-S clusters and interact with stromal HSP70B. Biochem J 427(2): 205-215.
  13. Armbruster, U., Zuhlke, J., Rengstl, B., Kreller, R., Makarenko, E., Ruhle, T., Schunemann, D., Jahns, P., Weisshaar, B., Nickelsen, J. and Leister, D. (2010). The Arabidopsis thylakoid protein PAM68 is required for efficient D1 biogenesis and photosystem II assembly. Plant Cell 22(10): 3439-3460.
  14. Schwarz, C. and Nickelsen, J. (2010) Enrichment of native, high molecular weight ribonucleoprotein complexes from chloroplasts by consecutive gel filtration steps. Endocyt. Cell Res. 20, 89-94.
  15. Schottkowski, M., Gkalympoudis, S., Tzekova, N., Stelljes, C., Schunemann, D., Ankele, E. and Nickelsen, J. (2009). Interaction of the periplasmic PratA factor and the PsbA (D1) protein during biogenesis of photosystem II in Synechocystis sp. PCC 6803. J Biol Chem 284(3): 1813-1819.
  16. Bohne, A.-V., Schwarz, C., Jalal, A., Ossenbühl, F. and Nickelsen, J. (2009) Control of organellar gene expression in Chlamydomonas – future perspectives -. Endocyt. Cell Res. 19, 70-80.
  17. Wobbe, L., Blifernez, O., Schwarz, C., Mussgnug, J. H., Nickelsen, J. and Kruse, O. (2009). Cysteine modification of a specific repressor protein controls the translational status of nucleus-encoded LHCII mRNAs in Chlamydomonas. Proc Natl Acad Sci U S A 106(32): 13290-13295.
  18. Schottkowski, M., Ratke, J., Oster, U., Nowaczyk, M. and Nickelsen, J. (2009). Pitt, a novel tetratricopeptide repeat protein involved in light-dependent chlorophyll biosynthesis and thylakoid membrane biogenesis in Synechocystis sp. PCC 6803. Mol Plant 2(6): 1289-1297.
  19. Wobbe, L., Schwarz, C., Nickelsen, J. and Kruse, O. (2008). Translational control of photosynthetic gene expression in phototrophic eukaryotes. Physiol Plant 133(3): 507-515.
  20. Komenda, J., Nickelsen, J., Tichy, M., Prasil, O., Eichacker, L. A. and Nixon, P. J. (2008). The cyanobacterial homologue of HCF136/YCF48 is a component of an early photosystem II assembly complex and is important for both the efficient assembly and repair of photosystem II in Synechocystis sp. PCC 6803. J Biol Chem 283(33): 22390-22399.
  21. Koop, H.-U., Herz, S., Golds, T. J. and Nickelsen, J. (2007) The genetic transformation of plastids. In: Cell and Molecular Biology of Plastids, R. Rock (ed) Topics Curr. Genet. 20, 457-510.
  22. Schwarz, C., Elles, I., Kortmann, J., Piotrowski, M. and Nickelsen, J. (2007). Synthesis of the D2 protein of photosystem II in Chlamydomonas is controlled by a high molecular mass complex containing the RNA stabilization factor Nac2 and the translational activator RBP40. Plant Cell 19(11): 3627-3639.
  23. Klinkert, B., Elles, I. and Nickelsen, J. (2006). Translation of chloroplast psbD mRNA in Chlamydomonas is controlled by a secondary RNA structure blocking the AUG start codon. Nucleic Acids Res 34(1): 386-394.
  24. Nickelsen, J., Nowaczyk, M. and Klinkert, B. (2006) Assembly factors of the photosynthetic machinery in cyanobacteria. Prog. Bot. 68, 57-79.
  25. Nickelsen, J. (2005) Chlamydomonas as a genetic model system. Prog. Botany 66, 68-89.
  26. Balczun, C., Bunse, A., Hahn, D., Bennoun, P., Nickelsen, J. and Kuck, U. (2005). Two adjacent nuclear genes are required for functional complementation of a chloroplast trans-splicing mutant from Chlamydomonas reinhardtii. Plant J 43(5): 636-648
  27. Pasch, J. C., Nickelsen, J. and Schunemann, D. (2005). The yeast split-ubiquitin system to study chloroplast membrane protein interactions. Appl Microbiol Biotechnol 69(4): 440-447.
  28. Mussgnug, J. H., Wobbe, L., Elles, I., Claus, C., Hamilton, M., Fink, A., Kahmann, U., Kapazoglou, A., Mullineaux, C. W., Hippler, M., Nickelsen, J., Nixon, P. J. and Kruse, O. (2005). NAB1 is an RNA binding protein involved in the light-regulated differential expression of the light-harvesting antenna of Chlamydomonas reinhardtii. Plant Cell 17(12): 3409-3421.
  29. Klinkert, B., Schwarz, C., Pohlmann, S. and Nickelsen, J. (2005) Correlation of mRNA levels and protein accumulation in a psbD promotor mutant of Chlamydomonas reinhardtii. Molec. Gen. Genom. 274, 637-643.
2 Protocols published
35S pulse Labelling of Chlamydomonas Chloroplast Proteins
Authors:  Alexandra-Viola Bohne, Christian Schwarz and Joerg Nickelsen, date: 06/05/2013, view: 9433, Q&A: 0
35S pulse labelling of proteins is used to attach a radioactive label to newly synthesized proteins, as sulfur is an element that is mainly present in proteins (Fleischmann and Rochaix 1999). Depending on your organism’s uptake mechanisms ...
2D Diagonal Redox SDS-PAGE of Proteins
Authors:  Christian Schwarz and Joerg Nickelsen, date: 06/05/2013, view: 14946, Q&A: 0
2D diagonal redox SDS-PAGE of proteins is used to detect intramolecular or intermolecular disulfide bridges using Chlamydomonas in this example (Stroeher and Dietz, 2008; Schwarz et al., 2012). Both dimensions consist of a ...
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