hi, I am trying some modifications of the above-mentioned protocol but still, I cant get a valid experimental setup. the problem is that both treated and control (no treated samples) give a fluorescence signal. another observation is that the fluorescence is visible even in the cell suspension so in this case, once I pellet down the E.coli cells and resuspend them in PBS (my working solution for flow cytometry) I have very weak or no signal. Could you please help me or test me if you have any recommendations?
thank you in advance
Maria
4/24/2018 3:29:49 AM Reply