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Coauthors
Kenta Goto Analytical Research Center for Experimental Sciences, Saga University, Japan
1 protocol

Yukio Nagano
  • Analytical Research Center for Experimental Sciences, Saga University, Japan
  • 1 Author merit

Education

Doctor of Agriculture, Graduate School of Agriculture, Kyoto University, Japan

Current position

Associate Professor, Analytical Research Center for Experimental Sciences, Saga University, Japan

Publications (since 2013)

  1. Goto, K. and Nagano, Y. (2013). Ultra-low background DNA cloning system. PLoS One 8(2): e56530.
  2. Penjor, T., Yamamoto, M., Uehara, M., Ide, M., Matsumoto, N., Matsumoto, R. and Nagano, Y. (2013). Phylogenetic relationships of citrus and its relatives based on matK gene sequences. PLoS One 8(4): e62574.
1 Protocol published
Ultra-low Background DNA Cloning System
Authors:  Yukio Nagano and Kenta Goto, date: 08/20/2013, view: 9570, Q&A: 0
We have developed a method to clone DNA fragments into the E. coli plasmid vectors with almost 100% efficiency (Goto and Nagano, 2013). This method is based on highly efficient yeast-based in vivo cloning, and the subsequent ...