Kentaro Kajiwara Department of Oncogene Research, Research Institute for Microbial Diseases, Osaka University, Japan
1 protocol

Takefumi Karashima Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University, Japan
1 protocol

Kouichi Funato
  • Department of Biofunctional Science and Technology, Graduate School of Biosphere Science, Hiroshima University, Japan
Research focus
  • Microbiology
  • 1 Author merit


Ph.D. in Graduate School of Pharmaceutical Sciences, University of Tokushima, 1994

Current position

Associate Professor in Graduate School of Biosphere Sciences, Hiroshima University


  1. Kajiwara, K., Muneoka, T., Watanabe, Y., Karashima, T., Kitagaki, H. and Funato, K. (2012). Perturbation of sphingolipid metabolism induces endoplasmic reticulum stress-mediated mitochondrial apoptosis in budding yeast. Mol Microbiol 86(5): 1246-1261.
  2. Kajiwara, K., Watanabe, R., Pichler, H., Ihara, K., Murakami, S., Riezman, H. and Funato, K. (2008). Yeast ARV1 is required for efficient delivery of an early GPI intermediate to the first mannosyltransferase during GPI assembly and controls lipid flow from the endoplasmic reticulum. Mol Biol Cell 19(5): 2069-2082.
  3. Funato, K. Riezman, H. (2006). Part 2-8, Sphingolipid Trafficking, HIRABAYASHI, Yoshio, IGARASHI, Yasuyuki, MERRILL, Alfred H. Jr, (Eds.), Sphingolipid Biology, pp.123-139, Springer.
  4. Funato, K., Lombardi, R., Vallee, B. and Riezman, H. (2003). Lcb4p is a key regulator of ceramide synthesis from exogenous long chain sphingoid base in Saccharomyces cerevisiae. J Biol Chem 278(9): 7325-7334.
  5. Funato, K., Vallee, B. and Riezman, H. (2002). Biosynthesis and trafficking of sphingolipids in the yeast Saccharomyces cerevisiae. Biochemistry 41(51): 15105-15114.
  6. Funato, K. and Riezman, H. (2001). Vesicular and nonvesicular transport of ceramide from ER to the Golgi apparatus in yeast. J Cell Biol 155(6): 949-959.
1 Protocol published
Authors:  Takefumi Karashima, Kentaro Kajiwara and Kouichi Funato, date: 08/20/2013, view: 8605, Q&A: 0
Yeast sphingolipids can be metabolically labeled with exogenously added radioactive precursors. Here we describe a method to label ceramides and complex sphingolipids of Saccharomyces cerevisiae with a radioactive ceramide precursor, ...
We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.