Edgar Nájera Departamento de Bioquímica, Universidad Nacional Autónoma de México, Mexico
1 protocol

Florencia García-Campusano Departamento de Bioquímica, Universidad Nacional Autónoma de México, Mexico
1 protocol

Lilia Bernal Departamento de Bioquímica, Universidad Nacional Autónoma de México, Mexico
1 protocol

Felipe Cruz-García
  • Departamento de Bioquímica, Universidad Nacional Autónoma de México, Mexico
  • 1 Author merit

Education

Ph.D. in Biochemistry, Faculty of Chemistry, National Autonomous University of Mexico, 2007

Current position

He is a professor in Faculty of Chemistry, National Autonomous University of Mexico. His research focuses on cell-to-cell recognition on pollen-pistil interactions

Selected publications

  1. Jimenez-Duran, K., McClure, B., Garcia-Campusano, F., Rodriguez-Sotres, R., Cisneros, J., Busot, G. and Cruz-Garcia, F. (2013). NaStEP: a proteinase inhibitor essential to self-incompatibility and a positive regulator of HT-B stability in Nicotiana alata pollen tubes. Plant Physiol 161(1): 97-107.
  2. Flores-Renteria, L., Orozco-Arroyo, G., Cruz-Garcia, F., Garcia-Campusano, F., Alfaro, I. and Vazquez-Santana, S. (2013). Programmed cell death promotes male sterility in the functional dioecious Opuntia stenopetala (Cactaceae). Ann Bot.
  3. Orozco-Arroyo, G., Vazquez-Santana, S., Camacho, A., Dubrovsky, J. G. and Cruz-Garcia, F. (2012). Inception of maleness: auxin contribution to flower masculinization in the dioecious cactus Opuntia stenopetala. Planta 236(1): 225-238.
  4. McClure, B., Cruz-Garcia, F. and Romero, C. (2011). Compatibility and incompatibility in S-RNase-based systems. Ann Bot 108(4): 647-658.
  5. Saucedo-Garcia, M., Guevara-Garcia, A., Gonzalez-Solis, A., Cruz-Garcia, F., Vazquez-Santana, S., Markham, J. E., Lozano-Rosas, M. G., Dietrich, C. R., Ramos-Vega, M., Cahoon, E. B. and Gavilanes-Ruiz, M. (2011). MPK6, sphinganine and the LCB2a gene from serine palmitoyltransferase are required in the signaling pathway that mediates cell death induced by long chain bases in Arabidopsis. New Phytol 191(4): 943-957. .
  6. Busot, G. Y., McClure, B., Ibarra-Sanchez, C. P., Jimenez-Duran, K., Vazquez-Santana, S. and Cruz-Garcia, F. (2008). Pollination in Nicotiana alata stimulates synthesis and transfer to the stigmatic surface of NaStEP, a vacuolar Kunitz proteinase inhibitor homologue. J Exp Bot 59(11): 3187-3201.
  7. Goldraij, A., Kondo, K., Lee, C. B., Hancock, C. N., Sivaguru, M., Vazquez-Santana, S., Kim, S., Phillips, T. E., Cruz-Garcia, F. and McClure, B. (2006). Compartmentalization of S-RNase and HT-B degradation in self-incompatible Nicotiana. Nature 439(7078): 805-810.
  8. Juarez-Diaz, J. A., McClure, B., Vazquez-Santana, S., Guevara-Garcia, A., Leon-Mejia, P., Marquez-Guzman, J. and Cruz-Garcia, F. (2006). A novel thioredoxin h is secreted in Nicotiana alata and reduces S-RNase in vitro. J Biol Chem 281(6): 3418-3424.
  9. Cruz-Garcia, F., Nathan Hancock, C., Kim, D. and McClure, B. (2005). Stylar glycoproteins bind to S-RNase in vitro. Plant J 42(3): 295-304.
1 Protocol published
Reverse Zymmogram Analysis for the Detection of Protease Inhibitor Activity
Authors:  Lilia Bernal, Florencia García-Campusano, Edgar Nájera and Felipe Cruz-García, date: 08/20/2013, view: 6587, Q&A: 0
This protocol describes a gel-based procedure to detect protease inhibitor activity. In this method gelatin is used as a substrate for proteolysis and is copolymerized within the polyacrylamide matrix. Protein extracts are fractioned by SDS-PAGE and ...
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