Cathy Yam Department of Comparative Medicine, University of Washington, USA
1 protocol

Adeline M. Hajjar
  • Department of Comparative Medicine, University of Washington, USA
Research focus
  • Immunology
  • 1 Author merit


Ph.D. in Microbiology, University of Washington, Seattle, 1995

Current position

Research Associate Professor, Department of Comparative Medicine, University of Washington, Seattle WA

Publications (since 2005)

  1. Hajjar, A. M.§, Ernst, R. K., Fortuno, E. S., 3rd, Brasfield, A. S., Yam, C. S., Newlon, L. A., Kollmann, T. R., Miller, S. I. and Wilson, C. B. (2012). Humanized TLR4/MD-2 mice reveal LPS recognition differentially impacts susceptibility to Yersinia pestis and Salmonella enterica. PLoS Pathog 8(10): e1002963.
  2. Awoniyi, M., Miller, S. I., Wilson, C. B., Hajjar, A. M.§ and Smith, K. D.§ (2012). Homeostatic regulation of Salmonella-induced mucosal inflammation and injury by IL-23. PLoS One 7(5): e37311.
  3. Kanistanon, D., Powell, D. A., Hajjar, A. M., Pelletier, M. R., Cohen, I. E., Way, S. S., Skerrett, S. J., Wang, X., Raetz, C. R. and Ernst, R. K. (2012). Role of Francisella lipid A phosphate modification in virulence and long-term protective immune responses. Infect Immun 80(3): 943-951.
  4. Corbett, N. P., Blimkie, D., Ho, K. C., Cai, B., Sutherland, D. P., Kallos, A., Crabtree, J., Rein-Weston, A., Lavoie, P. M., Turvey, S. E., Hawkins, N. R., Self, S. G., Wilson, C. B., Hajjar, A. M., Fortuno, E. S., 3rd and Kollmann, T. R. (2010). Ontogeny of Toll-like receptor mediated cytokine responses of human blood mononuclear cells. PLoS One 5(11): e15041.
  5. Marr, N., Novikov, A., Hajjar, A. M., Caroff, M. and Fernandez, R. C. (2010). Variability in the lipooligosaccharide structure and endotoxicity among Bordetella pertussis strains. J Infect Dis 202(12): 1897-1906.
  6. Marr, N., Hajjar, A. M., Shah, N. R., Novikov, A., Yam, C. S., Caroff, M. and Fernandez, R. C. (2010). Substitution of the Bordetella pertussis lipid A phosphate groups with glucosamine is required for robust NF-kappaB activation and release of proinflammatory cytokines in cells expressing human but not murine Toll-like receptor 4-MD-2-CD14. Infect Immun 78(5): 2060-2069.
  7. Parra, M. C., Shaffer, S. A., Hajjar, A. M., Gallis, B. M., Hager, A., Goodlett, D. R., Guina, T., Miller, S. and Collins, C. M. (2010). Identification, cloning, expression, and purification of Francisella lpp3: an immunogenic lipoprotein. Microbiol Res 165(7): 531-545.
  8. Aldape, M. J., Bryant, A. E., Katahira, E. J., Hajjar, A. M., Finegold, S. M., Ma, Y. and Stevens, D. L. (2010). Innate immune recognition of, and response to, Clostridium sordellii. Anaerobe 16(2): 125-130.
  9. Kollmann, T. R., Crabtree, J., Rein-Weston, A., Blimkie, D., Thommai, F., Wang, X. Y., Lavoie, P. M., Furlong, J., Fortuno, E. S., 3rd, Hajjar, A. M., Hawkins, N. R., Self, S. G. and Wilson, C. B. (2009). Neonatal innate TLR-mediated responses are distinct from those of adults. J Immunol 183(11): 7150-7160.
  10. Voronina, V. A., Takemaru, K., Treuting, P., Love, D., Grubb, B. R., Hajjar, A. M., Adams, A., Li, F. Q. and Moon, R. T. (2009). Inactivation of Chibby affects function of motile airway cilia. J Cell Biol 185(2): 225-233.
  11. Wurfel, M. M., Gordon, A. C., Holden, T. D., Radella, F., Strout, J., Kajikawa, O., Ruzinski, J. T., Rona, G., Black, R. A., Stratton, S., Jarvik, G. P., Hajjar, A. M., Nickerson, D. A., Rieder, M., Sevransky, J., Maloney, J. P., Moss, M., Martin, G., Shanholtz, C., Garcia, J. G., Gao, L., Brower, R., Barnes, K. C., Walley, K. R., Russell, J. A. and Martin, T. R. (2008). Toll-like receptor 1 polymorphisms affect innate immune responses and outcomes in sepsis. Am J Respir Crit Care Med 178(7): 710-720.
  12. Jansen, K., Blimkie, D., Furlong, J., Hajjar, A., Rein-Weston, A., Crabtree, J., Reikie, B., Wilson, C. and Kollmann, T. (2008). Polychromatic flow cytometric high-throughput assay to analyze the innate immune response to Toll-like receptor stimulation. J Immunol Methods 336(2): 183-192.
  13. West, T. E., Pelletier, M. R., Majure, M. C., Lembo, A., Hajjar, A. M. and Skerrett, S. J. (2008). Inhalation of Francisella novicida Delta mglA causes replicative infection that elicits innate and adaptive responses but is not protective against invasive pneumonic tularemia. Microbes Infect 10(7): 773-780.
  14. Lembo, A., Pelletier, M., Iyer, R., Timko, M., Dudda, J. C., West, T. E., Wilson, C. B., Hajjar, A. M. and Skerrett, S. J. (2008). Administration of a synthetic TLR4 agonist protects mice from pneumonic tularemia. J Immunol 180(11): 7574-7581.
  15. Kanistanon, D., Hajjar, A. M., Pelletier, M. R., Gallagher, L. A., Kalhorn, T., Shaffer, S. A., Goodlett, D. R., Rohmer, L., Brittnacher, M. J., Skerrett, S. J. and Ernst, R. K. (2008). A Francisella mutant in lipid A carbohydrate modification elicits protective immunity. PLoS Pathog 4(2): e24.
  16. Kollmann, T. R., Reikie, B., Blimkie, D., Way, S. S., Hajjar, A. M., Arispe, K., Shaulov, A. and Wilson, C. B. (2007). Induction of protective immunity to Listeria monocytogenes in neonates. J Immunol 178(6): 3695-3701.
  17. Skerrett, S. J., Wilson, C. B., Liggitt, H. D. and Hajjar, A. M. (2007). Redundant Toll-like receptor signaling in the pulmonary host response to Pseudomonas aeruginosa. American Journal of Physiology-Lung Cellular and Molecular Physiology 292(1): L312-L322.
  18. Hajjar, A. M., Harvey, M. D., Shaffer, S. A., Goodlett, D. R., Sjostedt, A., Edebro, H., Forsman, M., Bystrom, M., Pelletier, M., Wilson, C. B., Miller, S. I., Skerrett, S. J. and Ernst, R. K. (2006). Lack of in vitro and in vivo recognition of Francisella tularensis subspecies lipopolysaccharide by Toll-like receptors. Infect Immun 74(12): 6730-6738.
  19. Hajjar, A. M., Harowicz, H., Liggitt, H. D., Fink, P. J., Wilson, C. B. and Skerrett, S. J. (2005). An essential role for non-bone marrow-derived cells in control of Pseudomonas aeruginosa pneumonia. Am J Respir Cell Mol Biol 33(5): 470-475.
  20. Krishnegowda, G.*, Hajjar, A. M.*§, Zhu, J., Douglass, E. J., Uematsu, S., Akira, S., Woods, A. S. and Gowda, D. C.§(2005). Induction of proinflammatory responses in macrophages by the glycosylphosphatidylinositols of Plasmodium falciparum: cell signaling receptors, glycosylphosphatidylinositol (GPI) structural requirement, and regulation of GPI activity. J Biol Chem 280(9): 8606-8616.

    *Contributed equally, §contributing (or shared senior) author
1 Protocol published
Whole Spleen Flow Cytometry Assay
Authors:  Cathy S. Yam and Adeline M. Hajjar, date: 08/05/2013, view: 26230, Q&A: 0
In the Whole Spleen Flow Cytometry Assay, we used splenocytes directly ex vivo for stimulation with a variety of TLR ligands. The splenocytes were stimulated for a total of 4 hours, then stained for intracellular cytokines. We then examined ...
We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.