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A peer-reviewed protocol journal. No publication fee; no access fee.
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Ryutaro Utsumi
Bioscience Department, Kinki University, Japan
1 protocol
Eiji Ishii
Bioscience Department, Kinki University, Japan
1 protocol
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Adam Idoine
Carnegie Institution
13 protocols
Aksiniya Asenova
University of California, Davis
12 protocols
Andrea Puhar
The Laboratory for Molecular Infection Medicine Sweden (MIMS); Umeå Centre for Microbial Research (UCMR); Department of Molecular Biology, Umea University, Sweden
43 protocols
Arsalan Daudi
University of California
157 protocols
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Reviewer
Yoko Eguchi
Faculty, Kindai University
Research focus
Microbiology
Contributions
1
Author merit
Reviewer merit
Editor merit
1 Protocol published
Protein-peptide Interaction by Surface Plasmon Resonance
Authors:
Eiji Ishii
,
Yoko Eguchi
and
Ryutaro Utsumi
,
date:
02/05/2013,
view:
14772,
Q&A:
0
This protocol measures the protein-peptide interaction by surface plasmon resonance (SPR) using Biacore X100 (GE Healthcare). The Biacore system can monitor the direct interaction between biomolecules. There are several methods of immobilizing a ...
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7 Protocols reviewed
Gene Dosage Experiments in Enterobacteriaceae Using Arabinose-regulated Promoters
Authors:
Sanchari Bhattacharyya
,
Shimon Bershtein
and
Eugene I Shakhnovich
,
date:
07/20/2017,
view:
3478,
Q&A:
0
This protocol is used to assay the effect of protein over-expression on fitness of
E. coli
. It is based on a plasmid expression of a protein of interest from an arabinose-regulated pBAD promoter followed by the measurement of the ...
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Expression, Purification and Crystallisation of the Adenosine A
2A
Receptor Bound to an Engineered Mini G Protein
Authors:
Byron Carpenter
and
Christopher G. Tate
,
date:
04/20/2017,
view:
5441,
Q&A:
0
G protein-coupled receptors (GPCRs) promote cytoplasmic signalling by activating heterotrimeric G proteins in response to extracellular stimuli such as light, hormones and nucleosides. Structure determination of GPCR–G protein complexes is central ...
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