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A peer-reviewed protocol journal. No publication fee; no access fee.
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Ryutaro Utsumi
Bioscience Department, Kinki University, Japan
1 protocol
Eiji Ishii
Bioscience Department, Kinki University, Japan
1 protocol
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Adam Idoine
Carnegie Institution
14 protocols
Aksiniya Asenova
University of California, Davis
13 protocols
Andrea Puhar
The Laboratory for Molecular Infection Medicine Sweden (MIMS); Umeå Centre for Microbial Research (UCMR); Department of Molecular Biology, Umea University, Sweden
44 protocols
Arsalan Daudi
University of California
157 protocols
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Reviewer
Yoko Eguchi
Faculty, Kindai University
Research focus
Microbiology
Contributions
1
Author merit
Reviewer merit
Editor merit
1 Protocol published
Protein-peptide Interaction by Surface Plasmon Resonance
Authors:
Eiji Ishii
,
Yoko Eguchi
and
Ryutaro Utsumi
,
date:
02/05/2013,
view:
15416,
Q&A:
0
This protocol measures the protein-peptide interaction by surface plasmon resonance (SPR) using Biacore X100 (GE Healthcare). The Biacore system can monitor the direct interaction between biomolecules. There are several methods of immobilizing a ...
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8 Protocols reviewed
Optimized Protocol for the Incorporation of FDAA (HADA Labeling) for
in situ
Labeling of Peptidoglycan
Authors:
Katharina Peters
,
Manuel Pazos
,
Michael S. VanNieuwenhze
and
Waldemar Vollmer
,
date:
08/05/2019,
view:
675,
Q&A:
0
The essential peptidoglycan (PG) layer surrounds the cytoplasmic membrane in nearly all bacteria. It is needed to maintain the shape of the cell and protect it from lysis due to high turgor. Growth of the PG layer is a complex process that involves ...
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Gene Dosage Experiments in Enterobacteriaceae Using Arabinose-regulated Promoters
Authors:
Sanchari Bhattacharyya
,
Shimon Bershtein
and
Eugene I Shakhnovich
,
date:
07/20/2017,
view:
3861,
Q&A:
0
This protocol is used to assay the effect of protein over-expression on fitness of
E. coli
. It is based on a plasmid expression of a protein of interest from an arabinose-regulated pBAD promoter followed by the measurement of the ...
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