Search
Coauthors
Ryutaro Utsumi Bioscience Department, Kinki University, Japan
1 protocol

Eiji Ishii Bioscience Department, Kinki University, Japan
1 protocol
Coreviewers
Adam Idoine Carnegie Institution
2 protocols

Arsalan Daudi University of California
153 protocols

Claudia Catalanotti 10x Genomics
14 protocols

Fanglian He University of Pennsylvania
205 protocols

Reviewer
Yoko Eguchi
  • Faculty, Kindai University
Research focus
  • Microbiology
  • 1 Author merit
1 Protocol published
Protein-peptide Interaction by Surface Plasmon Resonance
Authors:  Eiji Ishii, Yoko Eguchi and Ryutaro Utsumi, date: 02/05/2013, view: 10504, Q&A: 0
This protocol measures the protein-peptide interaction by surface plasmon resonance (SPR) using Biacore X100 (GE Healthcare). The Biacore system can monitor the direct interaction between biomolecules. There are several methods of immobilizing a ...
7 Protocols reviewed
Gene Dosage Experiments in Enterobacteriaceae Using Arabinose-regulated Promoters
Authors:  Sanchari Bhattacharyya, Shimon Bershtein and Eugene I Shakhnovich, date: 07/20/2017, view: 960, Q&A: 0
This protocol is used to assay the effect of protein over-expression on fitness of E. coli. It is based on a plasmid expression of a protein of interest from an arabinose-regulated pBAD promoter followed by the measurement of the ...
More >
Expression, Purification and Crystallisation of the Adenosine A2A Receptor Bound to an Engineered Mini G Protein
Authors:  Byron Carpenter and Christopher G. Tate, date: 04/20/2017, view: 2223, Q&A: 0
G protein-coupled receptors (GPCRs) promote cytoplasmic signalling by activating heterotrimeric G proteins in response to extracellular stimuli such as light, hormones and nucleosides. Structure determination of GPCR–G protein complexes is central ...
More >