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Coauthors
Ryutaro Utsumi Bioscience Department, Kinki University, Japan
1 protocol

Eiji Ishii Bioscience Department, Kinki University, Japan
1 protocol
Coreviewers
Adam Idoine Carnegie Institution
10 protocols

Arsalan Daudi University of California
157 protocols

Claudia Catalanotti 10x Genomics
15 protocols

Fanglian He University of Pennsylvania
205 protocols

Reviewer
Yoko Eguchi
  • Faculty, Kindai University
Research focus
  • Microbiology
  • 1 Author merit
1 Protocol published
Protein-peptide Interaction by Surface Plasmon Resonance
Authors:  Eiji Ishii, Yoko Eguchi and Ryutaro Utsumi, date: 02/05/2013, view: 12067, Q&A: 0
This protocol measures the protein-peptide interaction by surface plasmon resonance (SPR) using Biacore X100 (GE Healthcare). The Biacore system can monitor the direct interaction between biomolecules. There are several methods of immobilizing a ...
7 Protocols reviewed
Gene Dosage Experiments in Enterobacteriaceae Using Arabinose-regulated Promoters
Authors:  Sanchari Bhattacharyya, Shimon Bershtein and Eugene I Shakhnovich, date: 07/20/2017, view: 1875, Q&A: 0
This protocol is used to assay the effect of protein over-expression on fitness of E. coli. It is based on a plasmid expression of a protein of interest from an arabinose-regulated pBAD promoter followed by the measurement of the ...
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Expression, Purification and Crystallisation of the Adenosine A2A Receptor Bound to an Engineered Mini G Protein
Authors:  Byron Carpenter and Christopher G. Tate, date: 04/20/2017, view: 3323, Q&A: 0
G protein-coupled receptors (GPCRs) promote cytoplasmic signalling by activating heterotrimeric G proteins in response to extracellular stimuli such as light, hormones and nucleosides. Structure determination of GPCR–G protein complexes is central ...
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