Yoko Eguchi —BIO-PROTOCOL

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Coauthors

Ryutaro Utsumi Bioscience Department, Kinki University, Japan
1 protocol

Eiji Ishii Bioscience Department, Kinki University, Japan
1 protocol

Coreviewers

Adam Idoine Carnegie Institution
11 protocols

Arsalan Daudi University of California
157 protocols

Claudia Catalanotti 10x Genomics
15 protocols

Fanglian He Bio-protocol
205 protocols

Reviewer

Yoko Eguchi

Faculty, Kindai University

Research focus

Microbiology

1 Author merit
Reviewer merit
Editor merit

1 Protocol published

Protein-peptide Interaction by Surface Plasmon Resonance

Authors: Eiji Ishii, Yoko Eguchi and Ryutaro Utsumi, date: 02/05/2013, view: 12503, Q&A: 0

This protocol measures the protein-peptide interaction by surface plasmon resonance (SPR) using Biacore X100 (GE Healthcare). The Biacore system can monitor the direct interaction between biomolecules. There are several methods of immobilizing a ...

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7 Protocols reviewed

Gene Dosage Experiments in Enterobacteriaceae Using Arabinose-regulated Promoters

Authors: Sanchari Bhattacharyya, Shimon Bershtein and Eugene I Shakhnovich, date: 07/20/2017, view: 2179, Q&A: 0

This protocol is used to assay the effect of protein over-expression on fitness of E. coli. It is based on a plasmid expression of a protein of interest from an arabinose-regulated pBAD promoter followed by the measurement of the ...

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Expression, Purification and Crystallisation of the Adenosine A_2A Receptor Bound to an Engineered Mini G Protein

Authors: Byron Carpenter and Christopher G. Tate, date: 04/20/2017, view: 3698, Q&A: 0

G protein-coupled receptors (GPCRs) promote cytoplasmic signalling by activating heterotrimeric G proteins in response to extracellular stimuli such as light, hormones and nucleosides. Structure determination of GPCR–G protein complexes is central ...

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