Frederic Bourgaud UMR1121 Laboratoire Agronomie et Environnement, Université de Lorraine-INRA, France
1 protocol

Alexandre Olry UMR1121 Laboratoire Agronomie et Environnement, Université de Lorraine-INRA, France
1 protocol

Guilhem Vialart UMR1121 Laboratoire Agronomie et Environnement, Université de Lorraine-INRA, France
1 protocol

Alain Hehn
  • UMR1121 Laboratoire Agronomie et Environnement, Université de Lorraine-INRA, France
  • 1 Author merit
Education

Ph D in molecualr and cellular biology (1995)

Current position

Associate professor at the Université de Lorraine (Nancy – France)

Publications

  1. Karamat, F., Olry, A., Doerper, S., Vialart, G., Ullmann, P., Werck-Reichhart, D., Bourgaud, F. and Hehn, A. (2012). CYP98A22, a phenolic ester 3'-hydroxylase specialized in the synthesis of chlorogenic acid, as a new tool for enhancing the furanocoumarin concentration in Ruta graveolens. BMC Plant Biol 12: 152.
  2. Vialart, G.*, Hehn, A., Olry, A., Ito, K., Krieger, C., Larbat, R., Paris, C., Shimizu, B., Sugimoto, Y., Mizutani, M. and Bourgaud, F. (2012). A 2-oxoglutarate-dependent dioxygenase from Ruta graveolens L. exhibits p-coumaroyl CoA 2'-hydroxylase activity (C2'H): a missing step in the synthesis of umbelliferone in plants. Plant J 70(3): 460-470.
  3. Biteau, F., Nisse, E., Hehn, A., Miguel, S., Hannewald, P. and Bourgaud, F. (2012). A rapid and efficient method for isolating high quality DNA from leaves of carnivorous plants from the Drosera genus. Mol Biotechnol 51(3): 247-253.
  4. Amor, I. L., Hehn, A., Guedone, E., Ghedira, K., Engasser, J. M., Chekir-Ghedrira, L. and Ghoul, M. (2010). Biotransformation of naringenin to eriodictyol by Saccharomyces cerevisiea functionally expressing flavonoid 3' hydroxylase. Nat Prod Commun 5(12): 1893-1898.
  5. Olsen, K. M., Hehn, A., Jugde, H., Slimestad, R., Larbat, R., Bourgaud, F. and Lillo, C. (2010). Identification and characterisation of CYP75A31, a new flavonoid 3'5'-hydroxylase, isolated from Solanum lycopersicum. BMC Plant Biol 10: 21.
  6. Jousse, C., Vu, T. D., Tran Tle, M., Al Balkhi, M. H., Molinie, R., Boitel-Conti, M., Pilard, S., Mathiron, D., Hehn, A., Bourgaud, F. and Gontier, E. (2010). Tropane alkaloid profiling of hydroponic Datura innoxia Mill. Plants inoculated with Agrobacterium rhizogenes. Phytochem Anal 21(1): 118-127.
  7. Matsuno, M., Compagnon, V., Schoch, G. A., Schmitt, M., Debayle, D., Bassard, J. E., Pollet, B., Hehn, A., Heintz, D., Ullmann, P., Lapierre, C., Bernier, F., Ehlting, J. and Werck-Reichhart, D. (2009). Evolution of a novel phenolic pathway for pollen development. Science 325(5948): 1688-1692.
  8. Comino, C., Hehn, A., Moglia, A., Menin, B., Bourgaud, F., Lanteri, S. and Portis, E. (2009). The isolation and mapping of a novel hydroxycinnamoyltransferase in the globe artichoke chlorogenic acid pathway. BMC Plant Biol 9: 30.
  9. Larbat, R., Hehn, A.#, Hans, J., Schneider, S., Jugde, H., Schneider, B., Matern, U. and Bourgaud, F. (2009). Isolation and functional characterization of CYP71AJ4 encoding for the first P450 monooxygenase of angular furanocoumarin biosynthesis. J Biol Chem 284(8): 4776-4785
    * How to cite this protocol: please cite the above paper that starts with *.
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1 Protocol published
Extraction of Coumarins from Leaves, Petioles, Stems and Roots of Ruta graveolens and Nicotiana benthamiana
Authors:  Alain Hehn, Guilhem Vialart, Alexandre Olry and Frederic Bourgaud, date: 11/20/2012, view: 6578, Q&A: 0
This method describes the extraction of coumarins and furanocoumarins from leaves of Ruta graveolens (a natural furanocoumarin) producer, and Nicotiana benthamiana.
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