Vanesa Olivares-Illana Instituto de Física, Universidad Autónoma de San Luis Potosí
2 protocols

Robin Farhaeus
  • Cibles Therapeutiques, INSERM Unite 940, Universite Paris 7, France
Research focus
  • Biochemistry
  • 2 Author merit
2 Protocols published
从哺乳动物细胞中纯化带His标签泛素化蛋白
Authors:  Vanesa Olivares Illana and Robin Farhaeus, date: 09/05/2012, view: 19879, Q&A: 10
This protocol is used to purify His-tag ubiquitin conjugated protein. In this particular case, cells were transfected with His-tag ubiquitin and p53 which allowed us to purify using His-tag and reveal the WB using antibodies against p53 to see just ...
蛋白质-RNA酶联免疫吸附试验
Authors:  Vanesa Olivares Illana and Robin Farhaeus, date: 09/05/2012, view: 12086, Q&A: 0
Protein-RNA ELISA assay is an effective and quantitative method to study protein-RNA interactions in vitro. In this protocol we used recombinant 6x HIS tagged protein, but it works as well for non tagged proteins.
We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.