Roman Sobotka Laboratory of Photosynthesis, Institute of Microbiology, Třeboň
3 protocols

Jana Kopečná Institute of Microbiology, Academy of Sciences, Czech Republic
2 protocols

Luděk Kořený Department of Molecular Parasitology, Institute of Parasitology, Biology Centre ASCR, Czech Republic
1 protocol

Miroslav Oborník
  • Department of Molecular Parasitology, Institute of Parasitology, Biology Centre ASCR, Czech Republic
Research focus
  • Biochemistry
  • 1 Author merit


Ph.D. in Plant Pathology, Department of Plant Production, Faculty of Agriculture, University of South Bohemia, Ceské Budějovice, Czech Republic, 1995

Current Position

Associate Professor in Molecular and Cell Biology and Genetics, Department of Molecular Biology, Faculty of Science, University of South Bohemia, Ceské Budějovice, Czech Republic.
Senior Research Scientist, Institute of Parasitology, Biology Centre of the Academy of Sciences of the Czech Republic, Ceské Budějovice, Czech Republic.
Senior Research Scientist, Institute of Microbiology, Academy of Sciences of the Czech Republic, Treboň, Czech Republic.

Publications (since 2005)

  1. Burki, F., Flegontov, P., Obornik, M., Cihlar, J., Pain, A., Lukes, J. and Keeling, P. J. (2012). Re-evaluating the green versus red signal in eukaryotes with secondary plastid of red algal origin. Genome Biol Evol 4(6): 626-635 .(IF= 2.674)
  2. Koreny, L., Sobotka, R., Kovarova, J., Gnipova, A., Flegontov, P., Horvath, A., Obornik, M., Ayala, F. J. and Lukes, J. (2012). Aerobic kinetoplastid flagellate Phytomonas does not require heme for viability. Proc Natl Acad Sci U S A 109(10): 3808-3813. (IF=9.771)
  3. Qablan, M. A., Sloboda, M., Jirku, M., Obornik, M., Dwairi, S., Amr, Z. S., Horin, P., Lukes, J. and Modry, D. (2012). Quest for the piroplasms in camels: identification of Theileria equi and Babesia caballi in Jordanian dromedaries by PCR. Vet Parasitol 186(3-4): 456-460. (IF= 2.331)
  4. Valencakova, A., Balent, P., Ravaszova, P., Horak, A., Obornik, M., Halanova, M., Malcekova, B., Novotny, F. and Goldova, M. (2012). Molecular identification and genotyping of Microsporidia in selected hosts. Parasitol Res 110(2): 689-693. (IF=1.812)
  5. Koreny, L., Sobotka, R., Janouskovec, J., Keeling, P. J. and Obornik, M. (2011). Tetrapyrrole synthesis of photosynthetic chromerids is likely homologous to the unusual pathway of apicomplexan parasites. Plant Cell 23(9): 3454-3462. (IF= 9.396)
  6. Koblizek, M., Janouskovec, J., Obornik, M., Johnson, J. H., Ferriera, S. and Falkowski, P. G. (2011). Genome sequence of the marine photoheterotrophic bacterium Erythrobacter sp. strain NAP1. J Bacteriol 193(20): 5881-5882. (IF=3.726)
  7. Allen, A. E., Dupont, C. L., Obornik, M., Horak, A., Nunes-Nesi, A., McCrow, J. P., Zheng, H., Johnson, D. A., Hu, H., Fernie, A. R. and Bowler, C. (2011). Evolution and metabolic significance of the urea cycle in photosynthetic diatoms. Nature 473(7346): 203-207. (IF= 36.104)
  8. Koreny, L. and Obornik, M. (2011). Sequence evidence for the presence of two tetrapyrrole pathways in Euglena gracilis. Genome Biol Evol 3: 359-364. (IF= 2.674)
  9. Borovička, J., Noordeloos, M. E., Gryndler, M. and Oborník, M. (2011). Molecular phylogeny of Psilocybe cyanescens complex in Europe, with reference to the position of the secotioid Weraroa novae-zelandiae. Mycological Progress 10(2): 149-155. (IF= 1.266)
  10. Obornik, M., Vancova, M., Lai, D. H., Janouskovec, J., Keeling, P. J. and Lukes, J. (2011). Morphology and ultrastructure of multiple life cycle stages of the photosynthetic relative of apicomplexa, Chromera velia. Protist 162(1): 115-130. (IF= 3.329)
  11. Jiroutova, K., Koreny, L., Bowler, C. and Obornik, M. (2010). A gene in the process of endosymbiotic transfer. PLoS One 5(10): e13234. (IF= 4.411)
  12. Janouskovec, J., Horak, A., Obornik, M., Lukes, J. and Keeling, P. J. (2010). A common red algal origin of the apicomplexan, dinoflagellate, and heterokont plastids. Proc Natl Acad Sci U S A 107(24): 10949-10954. (IF=9.771)
  13. Koreny, L., Lukes, J. and Obornik, M. (2010). Evolution of the haem synthetic pathway in kinetoplastid flagellates: an essential pathway that is not essential after all? Int J Parasitol 40(2): 149-156. (IF= 3.882)
  14. Poliak, P., Van Hoewyk, D., Obornik, M., Zikova, A., Stuart, K. D., Tachezy, J., Pilon, M. and Lukes, J. (2010). Functions and cellular localization of cysteine desulfurase and selenocysteine lyase in Trypanosoma brucei. FEBS J 277(2): 383-393. (IF= 3.129)
  15. Jirku, M., Jirku, M., Obornik, M., Lukes, J. and Modry, D. (2009). A model for taxonomic work on homoxenous coccidia: redescription, host specificity, and molecular phylogeny of Eimeria ranae Dobell, 1909, with a review of anuran-host Eimeria (Apicomplexa: Eimeriorina). J Eukaryot Microbiol 56(1): 39-51. (IF= 2.397)
  16. Jirku, M., Jirku, M., Obornik, M., Lukes, J. and Modry, D. (2009). Goussia Labbe, 1896 (Apicomplexa, Eimeriorina) in Amphibia: diversity, biology, molecular phylogeny and comments on the status of the genus. Protist 160(1): 123-136. (IF= 3.329)
  17. Obornik, M., Janouskovec, J., Chrudimsky, T. and Lukes, J. (2009). Evolution of the apicoplast and its hosts: from heterotrophy to autotrophy and back again. Int J Parasitol 39(1): 1-12. (IF= 3.882)
  18. Oborník M (2009) Identification and Diagnostics of Entomopathogenic Protozoa. in: SP Stock, J Vandenberg, I Glazer and Boemare N, (eds.) Insects Pathogens: Molecular Approaches and Techniques, CAB International, 101-128. (book chapter)
  19. Moore, R. B., Obornik, M., Janouskovec, J., Chrudimsky, T., Vancova, M., Green, D. H., Wright, S. W., Davies, N. W., Bolch, C. J., Heimann, K., Slapeta, J., Hoegh-Guldberg, O., Logsdon, J. M. and Carter, D. A. (2008). A photosynthetic alveolate closely related to apicomplexan parasites. Nature 451(7181): 959-963. (IF= 36.104)
  20. Coesel, S., Obornik, M., Varela, J., Falciatore, A. and Bowler, C. (2008). Evolutionary origins and functions of the carotenoid biosynthetic pathway in marine diatoms. PLoS One 3(8): e2896 .(IF= 4.411)
  21. Bowler, C., Allen, A. E., Badger, J. H., Grimwood, J., Jabbari, K., Kuo, A., Maheswari, U., Martens, C., Maumus, F., Otillar, R. P., Rayko, E., Salamov, A., Vandepoele, K., Beszteri, B., Gruber, A., Heijde, M., Katinka, M., Mock, T., Valentin, K., Verret, F., Berges, J. A., Brownlee, C., Cadoret, J. P., Chiovitti, A., Choi, C. J., Coesel, S., De Martino, A., Detter, J. C., Durkin, C., Falciatore, A., Fournet, J., Haruta, M., Huysman, M. J., Jenkins, B. D., Jiroutova, K., Jorgensen, R. E., Joubert, Y., Kaplan, A., Kroger, N., Kroth, P. G., La Roche, J., Lindquist, E., Lommer, M., Martin-Jezequel, V., Lopez, P. J., Lucas, S., Mangogna, M., McGinnis, K., Medlin, L. K., Montsant, A., Oudot-Le Secq, M. P., Napoli, C., Obornik, M., Parker, M. S., Petit, J. L., Porcel, B. M., Poulsen, N., Robison, M., Rychlewski, L., Rynearson, T. A., Schmutz, J., Shapiro, H., Siaut, M., Stanley, M., Sussman, M. R., Taylor, A. R., Vardi, A., von Dassow, P., Vyverman, W., Willis, A., Wyrwicz, L. S., Rokhsar, D. S., Weissenbach, J., Armbrust, E. V., Green, B. R., Van de Peer, Y. and Grigoriev, I. V. (2008). The Phaeodactylum genome reveals the evolutionary history of diatom genomes. Nature 456(7219): 239-244. (IF= 36.104)
  22. Jiroutova, K., Horak, A., Bowler, C. and Obornik, M. (2007). Tryptophan biosynthesis in stramenopiles: eukaryotic winners in the diatom complex chloroplast. J Mol Evol 65(5): 496-511. (IF= 2.311)
  23. Hylis, M., Obornik, M., Nebesarova, J. and Vavra, J. (2007). Aquatic tetrasporoblastic microsporidia from caddis flies (Insecta, Trichoptera): characterisation, phylogeny and taxonomic reevaluation of the genera Episeptum Larsson, 1986, Pyrotheca Hesse, 1935 and Cougourdella Hesse, 1935. Eur J Protistol 43(3): 205-224. (IF= 2.322)
  24. Lukes, J., Mauricio, I. L., Schonian, G., Dujardin, J. C., Soteriadou, K., Dedet, J. P., Kuhls, K., Tintaya, K. W., Jirku, M., Chocholova, E., Haralambous, C., Pratlong, F., Obornik, M., Horak, A., Ayala, F. J. and Miles, M. A. (2007). Evolutionary and geographical history of the Leishmania donovani complex with a revision of current taxonomy. Proc Natl Acad Sci U S A 104(22): 9375-9380. (IF=9.771)
  25. Svobodova, M., Zidkova, L., Cepicka, I., Obornik, M., Lukes, J. and Votypka, J. (2007). Sergeia podlipaevi gen. nov., sp. nov. (Trypanosomatidae, Kinetoplastida), a parasite of biting midges (Ceratopogonidae, Diptera). Int J Syst Evol Microbiol 57(Pt 2): 423-432. (IF=1.930)
  26. Kopecna, J., Jirku, M., Obornik, M., Tokarev, Y. S., Lukes, J. and Modry, D. (2006). Phylogenetic analysis of coccidian parasites from invertebrates: search for missing links. Protist 157(2): 173-183. (IF= 3.329)
  27. Machackova, M., Obornik, M. and Kopecky, J. (2006). Effect of salivary gland extract from Ixodes ricinus ticks on the proliferation of Borrelia burgdorferi sensu stricto in vivo. Folia Parasitol (Praha) 53(2): 153-158. (IF= 1.533)
  28. Hylis, M., Pilarska, D. K., Obornik, M., Vavra, J., Solter, L. F., Weiser, J., Linde, A. and McManus, M. L. (2006). Nosema chrysorrhoeae n. sp. (Microsporidia), isolated from browntail moth (Euproctis chrysorrhoea L.) (Lepidoptera, Lymantriidae) in Bulgaria: characterization and phylogenetic relationships. J Invertebr Pathol 91(2): 105-114. (IF= 2.049)
  29. Obornik, M. and Green, B. R. (2005). Mosaic origin of the heme biosynthesis pathway in photosynthetic eukaryotes. Mol Biol Evol 22(12): 2343-2353. (IF= 5.510)
  30. Ležalová, R., Tkadlec, E., Oborník, M., Šimek, J. and Honza, M. (2005). Should males come first? The relationship between offspring hatching order and sex in the black‐headed gull Larus ridibundus. Journal of Avian Biology 36(6): 478-483.(IF= 2.310)
  31. Hylis, M., Weiser, J., Obornik, M. and Vavra, J. (2005). DNA isolation from museum and type collection slides of microsporidia. J Invertebr Pathol 88(3): 257-260. (IF= 2.049)
  32. Vavra, J., Hylis, M., Obornik, M. and Vossbrinck, C. R. (2005). Microsporidia in aquatic microcrustacea: the copepod microsporidium Marssoniella elegans Lemmermann, 1900 revisited. Folia Parasitol (Praha) 52(1-2): 163-172. (IF= 1.533)
1 Protocol published
Radiolabeling of Chlorophyll by [14C]Glutamic Acid in vivo and Relative Quantification of Labeled Chlorophyll by Using Thin Layer Chromatography (TLC)
Authors:  Roman Sobotka, Luděk Kořený, Jana Kopečná and Miroslav Oborník, date: 08/20/2012, view: 7666, Q&A: 0
This is an accurate method to assess the rate of chlorophyll biosynthesis in vivo in cyanobacteria. Given that labeled glutamate is used as the very early precursor of chlorophyll together with a short pulse of labeling (30 min), this ...
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