Roman Sobotka Laboratory of Photosynthesis, Institute of Microbiology, Třeboň
3 protocols

Judith Noda Institute of Microbiology, Academy of Sciences, Czech Republic
1 protocol

Jan Pilný Institute of Microbiology, Academy of Sciences, Czech Republic
1 protocol

Miroslav Oborník Department of Molecular Parasitology, Institute of Parasitology, Biology Centre ASCR, Czech Republic
1 protocol

Jana Kopečná
  • Institute of Microbiology, Academy of Sciences, Czech Republic
Research focus
  • Biochemistry
  • 2 Author merit


Ph.D. in Molecular Biology and Biochemistry, Faculty of Science, University of South Bohemia, České Budějovice, Czech Republic, 2012

Current position

Postdoctoral Fellow in Laboratory of Photosynthesis, Institute of Microbiology in Trebon, Czech Academy of Sciences, Czech Republic (2012-present)
Research focus: Chlorophyll biosythesis in cyanobacteria, cyanobacterial physiology, protein-protein interactions and complexes, pigment analysis, basic enzymology.


  1. Kopečná, J., Sobotka, R. and Komenda, J. (2013). Inhibition of chlorophyll biosynthesis at the protochlorophyllide reduction step results in the parallel depletion of Photosystem I and Photosystem II in the cyanobacterium Synechocystis PCC 6803. Planta 237(2): 497-508.
  2. Kopečná, J., Komenda, J., Bucinska, L. and Sobotka, R. (2012). Long-term acclimation of the cyanobacterium Synechocystis sp. PCC 6803 to high light is accompanied by an enhanced production of chlorophyll that is preferentially channeled to trimeric photosystem I. Plant Physiol 160(4): 2239-2250.
  3. Hollingshead, S., Kopečná, J., Jackson, P. J., Canniffe, D. P., Davison, P. A., Dickman, M. J., Sobotka, R. and Hunter, C. N. (2012). Conserved chloroplast open-reading frame ycf54 is required for activity of the magnesium protoporphyrin monomethylester oxidative cyclase in Synechocystis PCC 6803. J Biol Chem 287(33): 27823-27833.
  4. Komenda, J., Knoppova, J., Kopečná, J., Sobotka, R., Halada, P., Yu, J., Nickelsen, J., Boehm, M. and Nixon, P. J. (2012). The Psb27 assembly factor binds to the CP43 complex of photosystem II in the cyanobacterium Synechocystis sp. PCC 6803. Plant Physiol 158(1): 476-486.
  5. Kubicek, K., Monnet, J., Scintilla, S., Kopečná, J., Arnesano, F., Trantirek, L., Chopard, C., Natile, G. and Kozelka, J. (2010). Unusual interstrand Pt (S,S-diaminocyclohexane)-GG crosslink formed by rearrangement of a classical intrastrand crosslink within a DNA duplex. Chem Asian J 5(2): 244-247.
  6. NECAS, P., SINDACO, R., KOŘENÝ, L. and KOPEČNÁ, J. (2009). Kinyongia asheorum sp. n., a new montane chameleon from the Nyiro Range, northern Kenya (Squamata: Chamaeleonidae). Zootaxa 2028: 41-50. (IF=0.853)
  7. Zikova, A., Kopečná, J., Schumacher, M. A., Stuart, K., Trantirek, L. and Lukes, J. (2008). Structure and function of the native and recombinant mitochondrial MRP1/MRP2 complex from Trypanosoma brucei. Int J Parasitol 38(8-9): 901-912.
  8. Kopečná, J., Jirku, M., Obornik, M., Tokarev, Y. S., Lukes, J. and Modry, D. (2006). Phylogenetic analysis of coccidian parasites from invertebrates: search for missing links. Protist 157(2): 173-183.
2 Protocols published
Detection and Quantification of Heme and Chlorophyll Precursors Using a High Performance Liquid Chromatography (HPLC) System Equipped with Two Fluorescence Detectors
Authors:  Jan Pilný, Jana Kopečná, Judith Noda and Roman Sobotka, date: 02/05/2015, view: 11742, Q&A: 0
Intermediates of tetrapyrrole biosynthetic pathway are low-abundant compounds, and their quantification is usually difficult, time consuming and requires large amounts of input material. Here, we describe a method allowing fast and accurate ...
Radiolabeling of Chlorophyll by [14C]Glutamic Acid in vivo and Relative Quantification of Labeled Chlorophyll by Using Thin Layer Chromatography (TLC)
Authors:  Roman Sobotka, Luděk Kořený, Jana Kopečná and Miroslav Oborník, date: 08/20/2012, view: 9050, Q&A: 0
This is an accurate method to assess the rate of chlorophyll biosynthesis in vivo in cyanobacteria. Given that labeled glutamate is used as the very early precursor of chlorophyll together with a short pulse of labeling (30 min), this ...
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