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Roman Sobotka
Laboratory of Photosynthesis, Institute of Microbiology, Třeboň
3 protocols
Jana Kopečná
Institute of Microbiology, Academy of Sciences, Czech Republic
2 protocols
Miroslav Oborník
Department of Molecular Parasitology, Institute of Parasitology, Biology Centre ASCR, Czech Republic
1 protocol
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Luděk Kořený
Department of Molecular Parasitology, Institute of Parasitology, Biology Centre ASCR, Czech Republic
Research focus
Biochemistry
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1
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1 Protocol published
Radiolabeling of Chlorophyll by [14C]Glutamic Acid
in vivo
and Relative Quantification of Labeled Chlorophyll by Using Thin Layer Chromatography (TLC)
Authors:
Roman Sobotka
,
Luděk Kořený
,
Jana Kopečná
and
Miroslav Oborník
,
date:
08/20/2012,
view:
7670,
Q&A:
0
This is an accurate method to assess the rate of chlorophyll biosynthesis
in vivo
in cyanobacteria. Given that labeled glutamate is used as the very early precursor of chlorophyll together with a short pulse of labeling (30 min), this ...
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