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Coauthors
Francesca Granucci Department of Biotechnology and Biosciences, University of Milano-Bicocca, Italy
2 protocols

Renato Ostuni Department of Biotechnology and Biosciences, University of Milano-Bicocca, Italy
2 protocols
Coreviewers
Achille Broggi Harvard Medical School
12 protocols

Alesssandro Arduini BROAD Institute of Harvard and MIT
2 protocols

Alexandros Alexandratos University of Athens
10 protocols

Andrea Puhar MIMS - Umea University
29 protocols

Editor
Ivan Zanoni
  • Assistant Professor, Harvard Medical School
Research focus
  • Immunology
  • Innate immunity, inflammation, signal transduction
  • 2 Author merit

Education

Ph.D. in Immunology

Lab information

http://www.childrenshospital.org/research-and-innovation/research/labs/zanoni-lab

Publications

https://www.ncbi.nlm.nih.gov/pubmed/?term=zanoni+ivan+%5Bauthor%5D

2 Protocols published
Generation of Mouse Bone Marrow-Derived Dendritic Cells (BM-DCs)
Authors:  Francesca Granucci, Renato Ostuni and Ivan Zanoni, date: 06/20/2012, view: 12070, Q&A: 0
Generating mouse dendritic cells from bone-marrow progenitor cells is a useful tool to study biological functions of mouse dendritic cells. Dendritic cells are one of the major populations of phagocytes able to activate both innate and adaptive ...
Generation of Mouse Bone Marrow-Derived Macrophages (BM-MFs)
Authors:  Ivan Zanoni, Renato Ostuni and Francesca Granucci, date: 06/20/2012, view: 9963, Q&A: 1
Generating mouse macrophages from bone-marrow progenitor cells is a useful tool to study biological functions of mouse macrophages. Macrophages are one of the major populations of phagocytes and play many different roles during inflammatory process ...
3 Protocols reviewed
Cytokine-stimulated Phosphoflow of PBMC Using CyTOF Mass Cytometry
Authors:  Rosemary Fernandez and Holden Maecker, date: 06/05/2015, view: 6334, Q&A: 1
Phosphorylation of tyrosine, serine, and threonine residues is critical for the control of protein activity involved in various cellular events. An assortment of kinases and phosphatases regulate intracellular protein phosphorylation in many ...
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Generation and Maturation of Human Monocyte-derived DCs
Authors:  Michela Spadaro, Monica Montone and Federica Cavallo, date: 08/05/2014, view: 10984, Q&A: 0
Dendritic cells (DC) are antigen-presenting cells, which play a critical role in the regulation of the adaptive immune response. They act as a bridge between the innate and the adaptive immune systems. An approach to study their function and ...
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69 Protocols edited
Visualization of RNA at the Single Cell Level by Fluorescent in situ Hybridization Coupled to Flow Cytometry
Authors:  Alice Coillard, Elodie Segura and Rajneesh, date: 06/20/2018, view: 63, Q&A: 0
The protocol described here has been developed to detect RNA at the single cell level. Fluorescent probes hybridize to target RNAs and are detected by flow cytometry after multiple amplification steps. Different types of RNA can be detected such as ...
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Quantification of Extracellular Double-stranded RNA Uptake and Subcellular Localization Using Flow Cytometry and Confocal Microscopy
Authors:  Tan A Nguyen, Lachlan Whitehead and Ken C Pang, date: 06/20/2018, view: 59, Q&A: 0
Double-stranded RNA is a potent pathogen-associated molecular pattern (PAMP) produced as a by-product of viral replication and a well-known hallmark of viral infection. Viral dsRNAs can be released from infected cells into the extracellular space ...
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