Alba Blesa Universidad Francisco de Vitoria
22 protocols

Li Zhang Li Zhang
2 protocols

Molly Leung University of British Columbia
2 protocols

Timo Lehti University of Helsinki
17 protocols

Reviewer
Zhenying Liu
  • Research scientist, University of Pennsylvania
Research focus
  • Microbiology
  • 1 Author merit
1 Protocol published
2 Protocols reviewed
Heterologous Expression and Purification of SARS-CoV2 Nucleocapsid Protein
Authors:  Ankur Garg, Lihong Liu, David D. Ho and Leemor Joshua-Tor, date: 08/05/2020, view: 1390, Q&A: 0
This protocol describes a step by step method for heterologous expression of SARS-CoV2 Nucleocapsid (N) protein in Escherichia coli. Moreover, this protocol includes steps to purify the N protein to high purity and homogeneity. Thus, ...
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A PhoA-STII Based Method for Efficient Extracellular Secretion and Purification of Fab from Escherichia coli
Authors:  Ziyan Wang, Yang Gao, Manyu Luo, Cedric Cagliero, Hua Jiang, Yueqing Xie, Jianwei Zhu and Huili Lu, date: 09/20/2019, view: 2612, Q&A: 0
In comparison with full-length IgGs, antigen binding fragments (Fabs) are smaller in size and do not require the complexed post-translational modification. Therefore, Fab can be cost-effectively produced using an Escherichia coli (E. coli ...
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