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Akira Karasawa
New York Structural Biology Center
2 protocols
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Toshimitsu Kawate
Molecular Medicine, Cornell University, USA, USA,
Research focus
Microbiology
Contributions
2
Author merit
Reviewer merit
Editor merit
2 Protocols published
High-throughput YO-PRO-1 Uptake Assay for P2X7 Receptors Expressed in HEK Cells
Authors:
Akira Karasawa
and
Toshimitsu Kawate
,
date:
07/20/2018,
view:
4251,
Q&A:
0
P2X7 receptors are extracellular ATP-gated ion channels that play broad physiological and pathological roles in animals (Sluyter, 2017). Activation of P2X7 receptors lead to the opening of membrane channels permeable for small cations like Na
+
...
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Expression and Purification of a Mammalian P2X7 Receptor from Sf9 Insect Cells
Authors:
Akira Karasawa
and
Toshimitsu Kawate
,
date:
09/05/2017,
view:
7406,
Q&A:
0
The P2X7 receptor is an extracellular ATP-gated ion channel found only in eukaryotes (Bartlett
et al
., 2014). Due to its unique properties among P2X receptors, such as formation of a large conductance pore, the P2X7 receptor has been ...
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