Toshimitsu Kawate —BIO-PROTOCOL

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Akira Karasawa Molecular Medicine, Cornell University, USA
2 protocols

Toshimitsu Kawate

Molecular Medicine, Cornell University, USA, USA,

2 Author merit
Reviewer merit
Editor merit

2 Protocols published

High-throughput YO-PRO-1 Uptake Assay for P2X7 Receptors Expressed in HEK Cells

Authors: Akira Karasawa and Toshimitsu Kawate, date: 07/20/2018, view: 2444, Q&A: 0

P2X7 receptors are extracellular ATP-gated ion channels that play broad physiological and pathological roles in animals (Sluyter, 2017). Activation of P2X7 receptors lead to the opening of membrane channels permeable for small cations like Na⁺ ...

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Expression and Purification of a Mammalian P2X7 Receptor from Sf9 Insect Cells

Authors: Akira Karasawa and Toshimitsu Kawate, date: 09/05/2017, view: 5509, Q&A: 0

The P2X7 receptor is an extracellular ATP-gated ion channel found only in eukaryotes (Bartlett et al., 2014). Due to its unique properties among P2X receptors, such as formation of a large conductance pore, the P2X7 receptor has been ...

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