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Felipe Trajtenberg Laboratory of Molecular & Structural Microbiology, Institut Pasteur de Montevideo, Uruguay, Uruguay,
1 protocol

Ariel Mechaly Laboratory of Molecular & Structural Microbiology, Institut Pasteur de Montevideo, Uruguay, Uruguay,
1 protocol

Nicole Larrieux Laboratory of Molecular & Structural Microbiology, Institut Pasteur de Montevideo, Uruguay, Uruguay,
1 protocol

Juan Imelio Laboratory of Molecular and Structural Microbiology, Institut Pasteur de Montevideo, Uruguay, Uruguay,
1 protocol

Alejandro Andrés Buschiazzo
  • Research scientist, Laboratory of Molecular & Structural Microbiology, Institut Pasteur de Montevideo, Uruguay, Uruguay,
Research focus
  • Biochemistry
  • bacterial signaling and regulation
    Leptospira
    spirochete motility
  • 1 Author merit
1 Protocol published
Snapshots of the Signaling Complex DesK:DesR in Different Functional States Using Rational Mutagenesis and X-ray Crystallography
We have developed protocols to generate site-specific variants of the histidine-kinase DesK and its cognate response regulator DesR, conducive to trapping different signaling states of the proteins. Co-expression of both partners in E. coli ...