Nigel Temperton —BIO-PROTOCOL

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Emanuele Montomoli 1 protocol

George Carnell University of Cambridge
2 protocols

Fabrizio Biuso 1 protocol

Eleonora Molesti 1 protocol

Nigel Temperton

Vial Pseudotype Unit, Medway School of Pharmacy, University of Kent, United Kingdom, United Kingdom,

Research focus

Microbiology

2 Author merit
Reviewer merit
Editor merit

Education

PhD, University of London, United Kingdom, 1998

Current position

Dr, Medway School of Pharmacy, University of Kent, United Kingdom

Publications

https://orcid.org/0000-0002-7978-3815

2 Protocols published

A Lentiviral Pseudotype ELLA for the Measurement of Antibodies Against Influenza Neuraminidase

Authors: Fabrizio Biuso, George Carnell, Emanuele Montomoli and Nigel Temperton, date: 07/20/2018, view: 3634, Q&A: 0

This protocol describes the rapid and safe production of lentiviral pseudotypes characterized by a lentiviral core containing a reporter, in conjunction with avian influenza haemagglutinin (HA) and human neuraminidase (NA) glycoproteins on the ...

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An Optimized Method for the Production Using PEI, Titration and Neutralization of SARS-CoV Spike Luciferase Pseudotypes

Authors: George Carnell, Keith Grehan, Francesca Ferrara, Eleonora Molesti and Nigel Temperton, date: 08/20/2017, view: 9535, Q&A: 1

The protocol outlined represents a cost-effective, rapid and reliable method for the generation of high-titre viral pseudotype particles with the wild-type SARS-CoV spike protein on a lentiviral vector core using the widely available transfection ...

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