Education

PhD, The University of Leicester, UK, 2005

Current position

Senior Scientist, Science and Technology Facilities Council, UK

Publications

1. Laura C. Zanetti-Domingues, Dimitrios Korovesis, Sarah R. Needham, Christopher J. Tynan, Shiori Sagawa, Selene K. Roberts, Antonija Kuzmanic, Elena Ortiz-Zapater, Purvi Jain, Rob C. Roovers, Alireza Lajevardipour, Paul M.P. van Bergen en Henegouwen, George Santis, Andrew H.A. Clayton, David T. Clarke, Francesco L. Gervasio, Yibing Shan, David E. Shaw, Daniel J. Rolfe, Peter J. Parker, and Marisa L. Martin-Fernandez. The architecture of EGFR's basal complexes reveals autoinhibition mechanisms in dimers and oligomers. Nat Comms (2018) DOI:10.1038/s41467-018-06632-0.
2. J. Claus, G. Patel, F. Autore, A. Colomba, Gregory Weitsman, Fanya N Soliman,  Selene Roberts, Laura C Zanetti-Domingues, Michael Hirsch, Francesca Collu, Roger George, Elena Ortiz-Zapater, Paul R Barber, Boris Vojnovic, Yosef Yarden, Marisa L Martin-Fernandez,   Angus Cameron,  Franca Fraternali, Tony Ng  and Peter J Parker. Inhibitor-induced HER2-HER3 heterodimerisation promotes proliferation through a novel dimer interface. eLife (2018);7:e32271 doi: 10.7554/eLife.32271
3. Needham, S.R., Roberts, S.K., Arkhipov, A., Mysore, V.P., Tynan, C.J., Zanetti-Domingues, L.C., Kim, E.T, Losasso, V., Korovesis, D., Hirsch, M., Rolfe, D.J., Clarke, D.T., Winn, M., Lajevardipour, A., Clayton, A.H.A. Pike, L.J., Perani, M., Parker, P.J., Shan, Y, Shaw, D.E. & Martin-Fernandez, M.L. EGFR oligomerization organizes kinase-active dimers into competent signalling platforms.  Nat Comms (2016) DOI: 10.1038/ncomms13307
   
4. Christopher J. Tynan, Valentina Lo Schiavo, Laura Zanetti-Domingues, Sarah R. Needham, Selene K. Roberts, Michael Hirsh, Daniel J. Rolfe, Dimitrios Korovesis, David T. Clarke and Marisa L. Martin-Fernandez.  A tale of the epidermal growth factor receptor: The quest for structural resolution on cells. Methods. (2015) DOI: 10.1016/j.ymeth.2015.10.009
5. Stephen E D Webb, Michael Hirsch, Sarah R Needham, Benji  C Coles, Selene K Roberts, Laura C Zanetti-Domingues, Chris J Tynan, Marisa L. Martin-Fernandez and Daniel J Rolfe. Nanometric molecular separation measurements by single molecule photobleaching. Methods (2015) DOI: 10.1016/j.ymeth.2015.05.009)
6. Sarah R. Needham, Laura C. Zanetti-Domingues, Kathrin Scherer, Michael Hirsch, Daniel J. Rolfe, Selene K. Roberts, Marisa L. Martin-Fernandez, David T. Clarke, Christopher J. Tynan. Determining the geometry of oligomers of the human epidermal growth factor family on cells with <10 nm resolution. Biochem. Soc. Trans. (2015) (3):309-14. doi: 10.1042/BST20140318.
7. Needham, S.R; Zanetti-Domingues, L.C; Hirsch, M., Rolfe, D. J; Tynan, C.J; Roberts, S K; Martin-Fernandez, M.L; Clarke, David T. Structure-function relationships and supramolecular organization of the EGFR (epidermal growth factor receptor) on the cell surface. Biochem. Soc. Trans. (2014) 42 Issue:1: 114-9 DOI:10.1042/BST20130236
8. L.C. Zanetti-Domingues, S.E.D. Webb, C. J. Tynan, D. J. Rolfe, M. Hirsch, B. Coles, S.R. Needham,  S. K. Roberts, D.T. Clarke and M. L. Martin-Fernandez. Simultaneous, multicolour single molecule imaging of the entire human epidermal growth factor receptor (HER) family in live breast cancer cells. Annals of Oncology (2012) doi.org/10.1093/annonc/mdt086.3.
9. S. K. Roberts, C.J. Tynan, M. Winn, and M.L. Martin-Fernandez. Investigating extracellular in situ EGFR structure and conformational changes using FRET microscopy. Biochem. Soc. Trans. (2012) 40(1):189-194.
10. D.T. Clarke, S.W. Botchway, B.C. Coles, S. R. Needham, S.K. Roberts, D.J. Rolfe, C.J. Tynan, A.D. Ward, S.E.D. Webb, R. Yadav, L. Zanetti-Domingues, and M.L. Martin-Fernandez. Optics clustered to output unique solutions: A multi-laser facility for combined single molecule and ensemble microscopy. Rev. Sci. Ins. (2011) 82, Article Number: 093705.
11. C.J. Tynan, S.K. Roberts, D.J. Rolfe, D.T. Clarke, H.H. Loeffler, J. Kästner, M.D. Winn P.J. Parker and M.L. Martin-Fernandez. Human epidermal growth factor receptor (EGFR) aligned on the plasma membrane adopts key features of drosophila EGFR asymmetry. Mol. Cell Biol. (2011) 31(11):2241-2252.
12. S. E. D. Webb, C.J. Tynan, L. Zanetti-Domingues, B.C. Coles, S.K. Roberts, D.J. Rolfe, S. R. Needham, M. Hirsch, D.T. Clarke, and M.L. Martin-Fernandez. Simultaneous, multicolour single molecule imaging of the entire ErbB receptor family in live cells. Eur. Biophys. J. with Biophys. Lett. (2011) 40(1): 154.
13. Kastner J., Loeffler H.H., Roberts S.K., Martin-Fernandez M.L., and Winn M.D. (2009). Ectodomain orientation, conformational plasticity and oligomerization of ErbB1 receptors investigated by molecular dynamics. J. Structural Biology (2009) 167(2):117-128.
14. S.E.D. Webb, D.J. Rolfe, S.R. Needham, S. K. Roberts, D.T. Clarke, C.I. McLachlan, M.P. Hobson and M.L. Martin-Fernandez. Simultaneous widefield single molecule orientation and FRET microscopy in cells. Optics Express (2008) 16(25): 20258-20265.
15. S.E.D. Webb, S. K. Roberts, S.R. Needham, C.J. Tynan, D.J. Rolfe, M.D. Winn, R. Barraclough, D.T. Clarke, and M.L. Martin-Fernandez. Single molecule imaging and FLIM show different structures for high and low-affinity EGFRs in A431 cells. Biophysics Journal (2008) 94:803-819.
16. Stephen E. D. Webb, Selene K. Roberts, Sarah R. Needham, Daniel J. Rolfe, Chris J. Tynan, and M.L. Martin-Fernandez. Epidermal growth factor signalling studied using multidimensional single molecule fluorescence microscopy. Proc. SPIE (2007) 6441, 64410E.
17. S.E.D. Webb, S.R. Needham, S.K. Roberts, and M.L. Martin-Fernandez. Multidimensional single molecule imaging in live cells using total internal reflection fluorescence microscopy. Optics Letters  (2006) 15;31(14):2157-9.
    
    Chapter in book:
    M.L. Martin-Fernandez, David T. Clarke, Sarah R. Needham, Selene K. Roberts, Daniel J. Rolfe, Chris J. Tynan, Stephen E.D. Webb and Martyn Winn. (2011). Investigating the conformation of membrane proteins in cells via single molecule microscopy. Molecular Imaging. ISBN 979-953-307-076-5.