Education

Ph.D. in Biochemistry, University of Glasgow, 1997

Current position

Research group leader in the CSIC (National Research Body in Spain), Estación Experimental del Zaidín (Granada, Spain)

Publications (since 2010)

1. Corral-Lugo, A., Daddaoua, A., Ortega, A., Espinosa-Urgel, M. and Krell, T. (2016). Rosmarinic acid is a homoserine lactone mimic produced by plants that activates a bacterial quorum-sensing regulator. Sci Signal 9(409): ra1.
   
2. Matilla, M. A., Nogellova, V., Morel, B., Krell, T. and Salmond, G. P. (2016). Biosynthesis of the acetyl-CoA carboxylase-inhibiting antibiotic, andrimid, in Serratia is regulated by Hfq and the LysR-type transcriptional regulator, AdmX. Environ Microbiol.
   
3. Corral-Lugo, A., de la Torre, J., Matilla, M. A., Fernandez, M., Morel, B., Espinosa-Urgel, M. and Krell, T. (2015). Assessment of the contribution of chemoreceptor-based signaling to biofilm formation. Environ Microbiol.
   
4. Martin-Mora, D., Reyes-Darias, J. A., Ortega, A., Corral-Lugo, A., Matilla, M. A. and Krell, T. (2015). McpQ is a specific citrate chemoreceptor that responds preferentially to citrate/metal ion complexes. Environ Microbiol.
   
5. Fernandez, M., Morel, B., Corral-Lugo, A. and Krell, T. (2016). Identification of a chemoreceptor that specifically mediates chemotaxis toward metabolizable purine derivatives. Mol Microbiol 99(1): 34-42.
   
6. Fernandez, M., Morel, B., Corral-Lugo, A., Rico-Jimenez, M., Martin-Mora, D., Lopez-Farfan, D., Reyes-Darias, J. A., Matilla, M. A., Ortega, A. and Krell, T. (2016). Identification of ligands for bacterial sensor proteins. Curr Genet 62(1): 143-147.
   
7. Reyes-Darias, J. A., Garcia, V., Rico-Jimenez, M., Corral-Lugo, A., Lesouhaitier, O., Juarez-Hernandez, D., Yang, Y., Bi, S., Feuilloley, M., Munoz-Rojas, J., Sourjik, V. and Krell, T. (2015). Specific gamma-aminobutyrate chemotaxis in pseudomonads with different lifestyle. Mol Microbiol 97(3): 488-501.
   
8. Krell, T. (2015). Tackling the bottleneck in bacterial signal transduction research: high-throughput identification of signal molecules. Mol Microbiol 96(4): 685-688.
   
9. Reyes-Darias, J. A., Yang, Y., Sourjik, V. and Krell, T. (2015). Correlation between signal input and output in PctA and PctB amino acid chemoreceptor of Pseudomonas aeruginosa. Mol Microbiol 96(3): 513-525.
   
10. Garcia, V., Reyes-Darias, J. A., Martin-Mora, D., Morel, B., Matilla, M. A. and Krell, T. (2015). Identification of a Chemoreceptor for C2 and C3 Carboxylic Acids. Appl Environ Microbiol 81(16): 5449-5457.
    
11. Silva-Jimenez, H., Ortega, A., Garcia-Fontana, C., Ramos, J. L. and Krell, T. (2015). Multiple signals modulate the activity of the complex sensor kinase TodS. Microb Biotechnol 8(1): 103-115.
    
12. Sampedro, I., Parales, R. E., Krell, T. and Hill, J. E. (2015). Pseudomonas chemotaxis. FEMS Microbiol Rev 39(1): 17-46.
    
13. Garcia-Fontana, C., Corral Lugo, A. and Krell, T. (2014). Specificity of the CheR2 methyltransferase in Pseudomonas aeruginosa is directed by a C-terminal pentapeptide in the McpB chemoreceptor. Sci Signal 7(320): ra34.
    
14. Daddaoua, A., Molina-Santiago, C., de la Torre, J., Krell, T. and Ramos, J. L. (2014). GtrS and GltR form a two-component system: the central role of 2-ketogluconate in the expression of exotoxin A and glucose catabolic enzymes in Pseudomonas aeruginosa. Nucleic Acids Res 42(12): 7654-7663.
    
15. Krell, T., Lacal, J., Garcia-Fontana, C., Silva-Jimenez, H., Rico-Jimenez, M., Lugo, A. C., Darias, J. A. and Ramos, J. L. (2014). Characterization of molecular interactions using isothermal titration calorimetry. Methods Mol Biol 1149: 193-203.
    
16. Darias, J. A., Garcia-Fontana, C., Lugo, A. C., Rico-Jimenez, M. and Krell, T. (2014). Qualitative and quantitative assays for flagellum-mediated chemotaxis. Methods Mol Biol 1149: 87-97.
    
17. Mayola, A., Irazoki, O., Martinez, I. A., Petrov, D., Menolascina, F., Stocker, R., Reyes-Darias, J. A., Krell, T., Barbe, J. and Campoy, S. (2014). RecA protein plays a role in the chemotactic response and chemoreceptor clustering of Salmonella enterica. PLoS One 9(8): e105578.
    
18. Chavarria, M., Durante-Rodriguez, G., Krell, T., Santiago, C., Brezovsky, J., Damborsky, J. and de Lorenzo, V. (2014). Fructose 1-phosphate is the one and only physiological effector of the Cra (FruR) regulator of Pseudomonas putida. FEBS Open Bio 4: 377-386.
    
19. Ortega-Gonzalez, M., Sanchez de Medina, F., Molina-Santiago, C., Lopez-Posadas, R., Pacheco, D., Krell, T., Martinez-Augustin, O. and Abdelali, D. (2014). Fructooligosacharides reduce Pseudomonas aeruginosa PAO1 pathogenicity through distinct mechanisms. PLoS One 9(1): e85772.
    
20. Molina-Santiago, C., Daddaoua, A., Fillet, S., Krell, T., Morel, B., Duque, E. and Ramos, J. L. (2014). Identification of new residues involved in intramolecular signal transmission in a prokaryotic transcriptional repressor. J Bacteriol 196(3): 588-594.
    
21. Ortega, A. and Krell, T. (2014). The HBM domain: introducing bimodularity to bacterial sensing. Protein Sci 23(3): 332-336.
    
22. Sevilla, E., Alvarez-Ortega, C., Krell, T. and Rojo, F. (2013). The Pseudomonas putida HskA hybrid sensor kinase responds to redox signals and contributes to the adaptation of the electron transport chain composition in response to oxygen availability. Environ Microbiol Rep 5(6): 825-834.
    
23. Rico-Jimenez, M., Munoz-Martinez, F., Krell, T., Gavira, J. A. and Pineda-Molina, E. (2013). Purification, crystallization and preliminary crystallographic analysis of the ligand-binding regions of the PctA and PctB chemoreceptors from Pseudomonas aeruginosa in complex with amino acids. Acta Crystallogr Sect F Struct Biol Cryst Commun 69(Pt 12): 1431-1435.
    
24. Daddaoua, A., Krell, T. and Ramos, J. L. (2013). Transcriptional control by two interacting regulatory proteins: identification of the PtxS binding site at PtxR. Nucleic Acids Res 41(22): 10150-10156.
    
25. Garcia-Fontana, C., Reyes-Darias, J. A., Munoz-Martinez, F., Alfonso, C., Morel, B., Ramos, J. L. and Krell, T. (2013). High specificity in CheR methyltransferase function: CheR2 of Pseudomonas putida is essential for chemotaxis, whereas CheR1 is involved in biofilm formation. J Biol Chem 288(26): 18987-18999.
    
26. Rico-Jimenez, M., Munoz-Martinez, F., Garcia-Fontana, C., Fernandez, M., Morel, B., Ortega, A., Ramos, J. L. and Krell, T. (2013). Paralogous chemoreceptors mediate chemotaxis towards protein amino acids and the non-protein amino acid gamma-aminobutyrate (GABA). Mol Microbiol 88(6): 1230-1243.
    
27. Sevilla, E., Silva-Jimenez, H., Duque, E., Krell, T. and Rojo, F. (2013). The Pseudomonas putida HskA hybrid sensor kinase controls the composition of the electron transport chain. Environ Microbiol Rep 5(2): 291-300.
    
28. Krell, T., Lacal, J., Reyes-Darias, J. A., Jimenez-Sanchez, C., Sungthong, R. and Ortega-Calvo, J. J. (2013). Bioavailability of pollutants and chemotaxis. Curr Opin Biotechnol 24(3): 451-456.
    
29. Lacal, J., Reyes-Darias, J. A., Garcia-Fontana, C., Ramos, J. L. and Krell, T. (2013). Tactic responses to pollutants and their potential to increase biodegradation efficiency. J Appl Microbiol 114(4): 923-933.
    
30. Pineda-Molina, E., Reyes-Darias, J. A., Lacal, J., Ramos, J. L., Garcia-Ruiz, J. M., Gavira, J. A. and Krell, T. (2012). Evidence for chemoreceptors with bimodular ligand-binding regions harboring two signal-binding sites. Proc Natl Acad Sci U S A 109(46): 18926-18931.
    
31. Pineda-Molina, E., Daddaoua, A., Krell, T., Ramos, J. L., Garcia-Ruiz, J. M. and Gavira, J. A. (2012). In situ X-ray data collection from highly sensitive crystals of Pseudomonas putida PtxS in complex with DNA. Acta Crystallogr Sect F Struct Biol Cryst Commun 68(Pt 11): 1307-1310.
    
32. Munoz-Martinez, F., Garcia-Fontana, C., Rico-Jimenez, M., Alfonso, C. and Krell, T. (2012). Genes encoding Cher-TPR fusion proteins are predominantly found in gene clusters encoding chemosensory pathways with alternative cellular functions. PLoS One 7(9): e45810.
    
33. Daddaoua, A., Fillet, S., Fernandez, M., Udaondo, Z., Krell, T. and Ramos, J. L. (2012). Genes for carbon metabolism and the ToxA virulence factor in Pseudomonas aeruginosa are regulated through molecular interactions of PtxR and PtxS. PLoS One 7(7): e39390.
    
34. Udaondo, Z., Duque, E., Fernandez, M., Molina, L., de la Torre, J., Bernal, P., Niqui, J. L., Pini, C., Roca, A., Matilla, M. A., Molina-Henares, M. A., Silva-Jimenez, H., Navarro-Aviles, G., Busch, A., Lacal, J., Krell, T., Segura, A. and Ramos, J. L. (2012). Analysis of solvent tolerance in Pseudomonas putida DOT-T1E based on its genome sequence and a collection of mutants. FEBS Lett 586(18): 2932-2938.
    
35. Santamaria-Hernando, S., Krell, T. and Ramos-Gonzalez, M. I. (2012). Identification of a novel calcium binding motif based on the detection of sequence insertions in the animal peroxidase domain of bacterial proteins. PLoS One 7(7): e40698.
    
36. Fillet, S., Daniels, C., Pini, C., Krell, T., Duque, E., Bernal, P., Segura, A., Lu, D., Zhang, X. and Ramos, J. L. (2012). Transcriptional control of the main aromatic hydrocarbon efflux pump in Pseudomonas. Environ Microbiol Rep 4(2): 158-167.
    
37. Gavira, J. A., Lacal, J., Ramos, J. L., Garcia-Ruiz, J. M., Krell, T. and Pineda-Molina, E. (2012). Crystallization and crystallographic analysis of the ligand-binding domain of the Pseudomonas putida chemoreceptor McpS in complex with malate and succinate. Acta Crystallogr Sect F Struct Biol Cryst Commun 68(Pt 4): 428-431.
    
38. Silva-Jimenez, H., Ramos, J. L. and Krell, T. (2012). Construction of a prototype two-component system from the phosphorelay system TodS/TodT. Protein Eng Des Sel 25(4): 159-169.
    
39. Krell, T., Lacal, J., Guazzaroni, M. E., Busch, A., Silva-Jimenez, H., Fillet, S., Reyes-Darias, J. A., Munoz-Martinez, F., Rico-Jimenez, M., Garcia-Fontana, C., Duque, E., Segura, A. and Ramos, J. L. (2012). Responses of Pseudomonas putida to toxic aromatic carbon sources. J Biotechnol 160(1-2): 25-32.
    
40. Arce-Rodriguez, A., Durante-Rodriguez, G., Platero, R., Krell, T., Calles, B. and de Lorenzo, V. (2012). The Crp regulator of Pseudomonas putida: evidence of an unusually high affinity for its physiological effector, cAMP. Environ Microbiol 14(3): 702-713.
    
41. Silva-Jimenez, H., Garcia-Fontana, C., Cadirci, B. H., Ramos-Gonzalez, M. I., Ramos, J. L. and Krell, T. (2012). Study of the TmoS/TmoT two-component system: towards the functional characterization of the family of TodS/TodT like systems. Microb Biotechnol 5(4): 489-500.
    
42. Segura, A., Molina, L., Fillet, S., Krell, T., Bernal, P., Munoz-Rojas, J. and Ramos, J. L. (2012). Solvent tolerance in Gram-negative bacteria. Curr Opin Biotechnol 23(3): 415-421.
    
43. Fillet, S., Krell, T., Morel, B., Lu, D., Zhang, X. and Ramos, J. L. (2011). Intramolecular signal transmission in a tetrameric repressor of the IclR family. Proc Natl Acad Sci U S A 108(37): 15372-15377.
    
44. Desbat, B., Lancelot, E., Krell, T., Nicolai, M. C., Vogel, F., Chevalier, M. and Ronzon, F. (2011). Effect of the β-propiolactone treatment on the adsorption and fusion of influenza A/Brisbane/59/2007 and A/New Caledonia/20/1999 virus H1N1 on a dimyristoylphosphatidylcholine/ganglioside GM3 mixed phospholipids monolayer at the air-water interface. Langmuir 27(22): 13675-13683.
    
45. Moreno, N., Chevalier, M., Ronzon, F., Manin, C., Dupuy, M., Krell, T. and Rieu, J. P. (2011). Unbinding forces of single pertussis toxin-antibody complexes measured by atomic force spectroscopy correlate with their dissociation rates determined by surface plasmon resonance. J Mol Recognit 24(6): 1105-1114.
    
46. Ramos, J. L., Marques, S., van Dillewijn, P., Espinosa-Urgel, M., Segura, A., Duque, E., Krell, T., Ramos-Gonzalez, M. I., Bursakov, S., Roca, A., Solano, J., Fernadez, M., Niqui, J. L., Pizarro-Tobias, P. and Wittich, R. M. (2011). Laboratory research aimed at closing the gaps in microbial bioremediation. Trends Biotechnol 29(12): 641-647.
    
47. Guichard, P., Krell, T., Chevalier, M., Vaysse, C., Adam, O., Ronzon, F. and Marco, S. (2011). Three dimensional morphology of rabies virus studied by cryo-electron tomography. J Struct Biol 176(1): 32-40.
    
48. Molina, L., Duque, E., Gomez, M. J., Krell, T., Lacal, J., Garcia-Puente, A., Garcia, V., Matilla, M. A., Ramos, J. L. and Segura, A. (2011). The pGRT1 plasmid of Pseudomonas putida DOT-T1E encodes functions relevant for survival under harsh conditions in the environment. Environ Microbiol 13(8): 2315-2327.
    
49. Lacal, J., Munoz-Martinez, F., Reyes-Darias, J. A., Duque, E., Matilla, M., Segura, A., Calvo, J. J., Jimenez-Sanchez, C., Krell, T. and Ramos, J. L. (2011). Bacterial chemotaxis towards aromatic hydrocarbons in Pseudomonas. Environ Microbiol 13(7): 1733-1744.
    
50. Lacal, J., Garcia-Fontana, C., Callejo-Garcia, C., Ramos, J. L. and Krell, T. (2011). Physiologically relevant divalent cations modulate citrate recognition by the McpS chemoreceptor. J Mol Recognit 24(2): 378-385.
    
51. Chavarria, M., Santiago, C., Platero, R., Krell, T., Casasnovas, J. M. and de Lorenzo, V. (2011). Fructose 1-phosphate is the preferred effector of the metabolic regulator Cra of Pseudomonas putida. J Biol Chem 286(11): 9351-9359.
    
52. Krell, T., Lacal, J., Munoz-Martinez, F., Reyes-Darias, J. A., Cadirci, B. H., Garcia-Fontana, C. and Ramos, J. L. (2011). Diversity at its best: bacterial taxis. Environ Microbiol 13(5): 1115-1124.
    
53. Lu, D., Fillet, S., Meng, C., Alguel, Y., Kloppsteck, P., Bergeron, J., Krell, T., Gallegos, M. T., Ramos, J. and Zhang, X. (2010). Crystal structure of TtgV in complex with its DNA operator reveals a general model for cooperative DNA binding of tetrameric gene regulators. Genes Dev 24(22): 2556-2565.
    
54. Lacal, J., Garcia-Fontana, C., Munoz-Martinez, F., Ramos, J. L. and Krell, T. (2010). Sensing of environmental signals: classification of chemoreceptors according to the size of their ligand binding regions. Environ Microbiol 12(11): 2873-2884.
    
55. Daniels, C., Krell, T., Michan, C. and Ramos, J. L. (2010). Struggling to get a universal meningococcal vaccine and novel uses for bacterial toxins in cancer treatment. Microb Biotechnol 3(4): 359-361.
    
56. Lacal, J., Alfonso, C., Liu, X., Parales, R. E., Morel, B., Conejero-Lara, F., Rivas, G., Duque, E., Ramos, J. L. and Krell, T. (2010). Identification of a chemoreceptor for tricarboxylic acid cycle intermediates: differential chemotactic response towards receptor ligands. J Biol Chem 285(30): 23126-23136.
    
57. Busch, A., Lacal, J., Silva-Jimenez, H., Krell, T. and Ramos, J. L. (2010). Catabolite repression of the TodS/TodT two-component system and effector-dependent transphosphorylation of TodT as the basis for toluene dioxygenase catabolic pathway control. J Bacteriol 192(16): 4246-4250.
    
58. Daddaoua, A., Krell, T., Alfonso, C., Morel, B. and Ramos, J. L. (2010). Compartmentalized glucose metabolism in Pseudomonas putida is controlled by the PtxS repressor. J Bacteriol 192(17): 4357-4366.
    
59. Krell, T., Lacal, J., Busch, A., Silva-Jimenez, H., Guazzaroni, M. E. and Ramos, J. L. (2010). Bacterial sensor kinases: diversity in the recognition of environmental signals. Annu Rev Microbiol 64: 539-559.