Li Feng
  • University of Wisconsin-Madison
Research focus
  • Stem cell
Cell Surface Protein Biotinylation and Analysis
A great way to specifically isolate and quantify proteins in the cell surface membrane is to take advantage of the biotinylation technique. It consists of labeling cell surface proteins with a biotin reagent before lysing the cells, and isolating these tagged proteins by NeutrAvidin pull-down. Then, the samples are subjected to SDS-PAGE ...
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