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matthias edmund birkicht

Center for Tropical marine research

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Biochemistry

Extraction and Molybdenum Blue-based Quantification of Total Phosphate and Polyphosphate in Parachlorella

Authors: Shuhei Ota and Shigeyuki Kawano, date: 09/05/2017, view: 9988, Q&A: 0

Inorganic phosphorus is a non-renewable resource and an essential element for life on Earth. Organisms such as algae, protists, and animals can store phosphate (Pi) through uptake of Pi as polyphosphate (poly-P), which is a linear polymer of orthophosphate residues linked by high-energy phosphoanhydride bonds. Here, we describe procedures for extraction of total phosphate and poly-P from Parachlorella cells and quantification of orthophosphate based on molybdenum blue assay. The present method may be applicable for other microalgae.

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Determination of total phosphorus in macro algae

[Feedback 1] We used 100 mg of dried macro algae tissue to determine the total phosphorus content. The powdered material was extracted in acidic K2S2O8 solution for 30 minutes at 120 degree celsius under pressure. After cooling an aliquot of the extract was filtered and analyzed according to the protocol with 96er wells at 810nm with a TECAN plate reader infinite F200Pro. The detection limit is 0.05 uM Phosphorus and the range is from 0.1 - 26.0 uM P. The relative standard deviation is better than 2% dörthe whole range.