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LL
Lin Luo
Vertax Pharmaceuticals
Research fields
Neuroscience
Isolation and Primary Cell Culture of Mouse Dorsal Root Ganglion Neurons
Authors:
Theresa Heinrich
,
Christian A. Hübner
and
Ingo Kurth
,
date:
04/05/2016,
view:
29922,
Q&A:
0
We here provide a detailed protocol for the isolation and culture of primary mouse sensory neurons. The cell bodies of sensory afferent pseudounipolar neurons are located in dorsal root ganglia (DRGs) along the vertebral column. Dissected mouse DRGs can be dissociated into single cells by enzymatic digestion to obtain primary cultures of mouse sensory neurons as performed in the studies reported by Khaminets
et al.
(2015).
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