B. Poovaiah Graduate Program in Molecular Plant Sciences, Washington State University, USA
1 protocol

Liqun Du Department of Horticulture, Washington State University, USA
1 protocol

Lei Zhang
  • Washington State University
Research focus
  • Plant science
  • 1 Author merit
In situ Hybridization (ISH) in Preparasitic and Parasitic Stages of the Plant-parasitic Nematode Meloidogyne spp.
The spatio-temporal expression pattern of a gene provides important indications to better understand its biological function. In situ hybridization (ISH) uses a labeled complementary single-stranded RNA or DNA probe to localize gene transcripts in a whole organism, a whole organ or a section of tissue. We adapted the ISH technique to the ...
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Formaldehyde for fixing nematodes
[Feedback 1] Since the formaldehyde recommended in the protocol has been discontinued by Sigma, I tried a similar product from sigma: 37% formaldehyde solution (Sigma: 252549), and it worked well for the in situ hybridization.

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