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Unbiased and Tailored CRISPR/Cas gRNA Libraries by Synthesizing Covalently-closed-circular (3Cs) DNA

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Bio-protocol publishes high quality, step-by-step protocols across the life sciences.

Biochemistry Biophysics Cancer Biology Cell Biology Developmental Biology Immunology Microbiology Molecular Biology Neuroscience Plant Science Stem Cell Systems Biology

Recognizing the need for greater reproducibility in scientific research, our collaborating journals encourage their
authors to publish step-by-step protocols in Bio-protocol.

Gregory Finnigan

Professor at Kansas State University

"The reviews we received from Bio-protocol were very insightful and greatly improved the clarity of our protocols."

Murugappan Sathappa

Research scientist at the Broad Institute of MIT and Harvard

"I truly appreciate the mission of Bio-protocol to publish protocols that can be reproduced by others."

Meenal Sinha

Associate specialist at UCSF

"Reviewing for Bio-Protocol has been very rewarding on multiple levels. The Bio-Protocol team is very professional and efficient."

New: Analysis of Monocyte Cell Fate by Adoptive Transfer in a Murine Model of TLR7-induced Systemic Inflammation http://bio-protocol.org/e4007 From Jaba Gamrekelashvili @jeckjones et al. #immunology #protocol @eLife

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New: Isolation of GFP-expressing Malarial Hypnozoites by Flow Cytometry Cell Sorting http://bio-protocol.org/e4006 #microbiology #protocol
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New: #COVID-19 Sample Pooling: From RNA Extraction to Quantitative Real-time RT-PCR http://bio-protocol.org/e4005 #virus #protocol @IMUMalaysia @plosone

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