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Unbiased and Tailored CRISPR/Cas gRNA Libraries by Synthesizing Covalently-closed-circular (3Cs) DNA

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Bio-protocol publishes high quality, step-by-step protocols across the life sciences.

Biochemistry Biophysics Cancer Biology Cell Biology Developmental Biology Immunology Microbiology Molecular Biology Neuroscience Plant Science Stem Cell Systems Biology

Recognizing the need for greater reproducibility in scientific research, our collaborating journals encourage their
authors to publish step-by-step protocols in Bio-protocol.

Gregory Finnigan

Professor at Kansas State University

"The reviews we received from Bio-protocol were very insightful and greatly improved the clarity of our protocols."

Murugappan Sathappa

Research scientist at the Broad Institute of MIT and Harvard

"I truly appreciate the mission of Bio-protocol to publish protocols that can be reproduced by others."

Meenal Sinha

Associate specialist at UCSF

"Reviewing for Bio-Protocol has been very rewarding on multiple levels. The Bio-Protocol team is very professional and efficient."

New: Development of a Chemical Reproductive Aging Model in Female Rats http://bio-protocol.org/e3994 #development #protocol @VBSDept #eNeuro

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New: Single Cell Analysis and Sorting of Aspergillus fumigatus by Flow Cytometry http://bio-protocol.org/e3993 #microbiology #protocol @MccirUoM @mbiojournal

Twitter • 15 hours ago

New: Measuring Cytosolic Translocation of Mycobacterium marinum in RAW264.7 Macrophages with a CCF4-AM FRET Assay http://bio-protocol.org/e3991 #microbiology #protocol @jbiolchem

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