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Detection of mRNA by Whole Mount in situ Hybridization and DNA Extraction for Genotyping of Zebrafish Embryos

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Bio-protocol publishes high quality, step-by-step protocols across the life sciences.

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Recognizing the need for greater reproducibility in scientific research, our collaborating journals encourage their
authors to publish step-by-step protocols in Bio-protocol.

Gregory Finnigan

Professor at Kansas State University

"The reviews we received from Bio-protocol were very insightful and greatly improved the clarity of our protocols."

Murugappan Sathappa

Research scientist at the Broad Institute of MIT and Harvard

"I truly appreciate the mission of Bio-protocol to publish protocols that can be reproduced by others."

Meenal Sinha

Associate specialist at UCSF

"Reviewing for Bio-Protocol has been very rewarding on multiple levels. The Bio-Protocol team is very professional and efficient."

New: Molecular Size Analysis of Recombinant Importin-histone Complexes Using Analytical Ultracentrifugation http://bio-protocol.org/e3625 Does not use any reporter tags or interaction with column resin thereby analyzing the interactions of the native proteins.
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@eLife

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New: Bacterial Lawn Avoidance and Bacterial Two Choice Preference Assays in Caenorhabditis elegans http://bio-protocol.org/e3623 These assays can be used in discovering various mechanisms of host learning that result in the avoidance of pathogens.
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@eLife

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New: Evaluation of the Efficiency of Genome Editing Tools by a Frameshift Fluorescence Protein Reporter http://bio-protocol.org/e3622 This #method can be adapted to optimize the genome-editing enzyme or the other components of the machinery. #cancer @umiamimedicine @umiamihealth

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