Protocols in Current Issue
    Behavioral Assays to Study Oxygen and Carbon Dioxide Sensing in Caenorhabditis elegans
    Authors:  Teresa Rojo Romanos, Leelee Ng, Manuel Zimmer and Roger Pocock, date: 01/05/2018, view: 192, Q&A: 0
    [Abstract] Animals use behavioral strategies to seek optimal environments. Population behavioral assays provide a robust means to determine the effect of genetic perturbations on the ability of animals to sense and respond to changes in the environment. Here, we describe a C. elegans population behavioral assay used to measure locomotory responses ...
    Infection of Caenorhabditis elegans with Vesicular Stomatitis Virus via Microinjection
    Authors:  Adam Martin, Emily A. Rex, Takao Ishidate, Rueyling Lin and Don B. Gammon, date: 11/20/2017, view: 507, Q&A: 0
    [Abstract] Over the past 15 years, the free-living nematode, Caenorhabditis elegans has become an important model system for exploring eukaryotic innate immunity to bacterial and fungal pathogens. More recently, infection models using either natural or non-natural nematode viruses have also been established in C. elegans. These models offer ...
    Nematode Epicuticle Visualisation by PeakForce Tapping Atomic Force Microscopy
    Authors:  Farida Akhatova, Gölnur Fakhrullina, Elvira Gayazova and Rawil Fakhrullin, date: 11/05/2017, view: 519, Q&A: 0
    [Abstract] The free-living soil nematode Caenorhabditis elegans has become an iconic experimental model animal in biology. This transparent animal can be easily imaged using optical microscopy to visualise its organs, tissues, single cells and subcellular events. The epicuticle of C. elegans nematodes has been studied at nanoscale using ...
    Generation of Caenorhabditis elegans Transgenic Animals by DNA Microinjection
    Authors:  Matthias Rieckher and Nektarios Tavernarakis, date: 10/05/2017, view: 999, Q&A: 0
    [Abstract] Microinjection is the most frequently used tool for genetic transformation of the nematode Caenorhabditis elegans, facilitating the transgenic expression of genes, genome editing by the clustered regularly interspersed short palindromic repeats (CRISPR)-Cas9 system, or transcription of dsRNA for RNA intereference (RNAi). Exogenous DNA is ...
    Lipidomic Analysis of Caenorhabditis elegans Embryos
    Authors:  Hung-Chi Yang, Cheng-Yu Hung, Yi-Yun Pan, Szecheng J Lo and Daniel Tsun-Yee Chiu, date: 09/20/2017, view: 1012, Q&A: 0
    [Abstract] Metabolomic is an emerging field of system biology. Lipidomic, a branch of metabolomic, aims to characterize lipophilic metabolites in biological systems. Caenorhabditis elegans (C. elegans) is a genetically tractable and versatile animal model for novel discovery of lipid metabolism. In addition, C. elegans embryo is ...
    Superoxide Dismutase (SOD) and Catalase (CAT) Activity Assay Protocols for Caenorhabditis elegans
    Authors:  Jing Zhang, Rui Chen, Zhenyang Yu and Lili Xue, date: 08/20/2017, view: 1472, Q&A: 0
    [Abstract] Assays for superoxide dismutase (SOD) and catalase (CAT) activities are widely employed to indicate antioxidant responses underlying the toxic effects of test chemicals. Yet, earlier studies mainly described the procedures as performed according to manufacturer’s instructions without modifications that are specific to any organisms. The present ...
    Primary Culture System for Germ Cells from Caenorhabditis elegans Tumorous Germline Mutants
    Authors:  Alexandra S. Vagasi, Mohammad M. Rahman, Snehal N. Chaudhari and Edward T. Kipreos, date: 08/05/2017, view: 1225, Q&A: 0
    [Abstract] The Caenorhabditis elegans germ line is an important model system for the study of germ stem cells. Wild-type C. elegans germ cells are syncytial and therefore cannot be isolated in in vitro cultures. In contrast, the germ cells from tumorous mutants can be fully cellularized and isolated intact from the mutant animals. ...
    Separation and Purification of Glycosaminoglycans (GAGs) from Caenorhabditis elegans
    Authors:  Tabea Dierker and Lena Kjellén, date: 08/05/2017, view: 1221, Q&A: 0
    [Abstract] The nematode Caenorhabditis elegans is a popular model organism for studies of developmental biology, neurology, ageing and other fields of basic research. Because many developmental processes are regulated by glycosaminoglyans (GAGs) on cell surfaces and in the extracellular matrix, methods to isolate and analyze C. elegans GAGs ...
    Aldicarb-induced Paralysis Assay to Determine Defects in Synaptic Transmission in Caenorhabditis elegans
    Authors:  Kelly H. Oh and Hongkyun Kim, date: 07/20/2017, view: 1052, Q&A: 0
    [Abstract] Aldicarb treatment causes an accumulation of acetylcholine in the synaptic cleft of the neuromuscular junction, resulting in sustained muscle activation and eventually paralysis. Aldicarb-induced paralysis assay is an easy and fast method to determine whether synaptic transmission of a C. elegans mutant of interest is altered. This assay ...
    Oxidative Stress Assays (arsenite and tBHP) in Caenorhabditis elegans
    Authors:  Collin Yvès Ewald, John M. Hourihan and T. Keith Blackwell, date: 07/05/2017, view: 1870, Q&A: 0
    [Abstract] Cells and organisms face constant exposure to reactive oxygen species (ROS), either from the environment or as a by-product from internal metabolic processes. To prevent cellular damage from ROS, cells have evolved detoxification mechanisms. The activation of these detoxification mechanisms and their downstream responses represent an overlapping ...