Protocols in Current Issue
    Magnetic Resonance Imaging and Histopathological Visualization of Human Dural Lymphatic Vessels
    Authors:  Seung-Kwon Ha, Govind Nair, Martina Absinta, Nicholas J. Luciano and Daniel S. Reich, date: 04/20/2018, view: 1013, Q&A: 0
    [Abstract] In this protocol, we describe a method to visualize and map dural lymphatic vessels in-vivo using magnetic resonance imaging (MRI) and ex-vivo using histopathological techniques. While MRI protocols for routine imaging of meningeal lymphatics include contrast-enhanced T2-FLAIR and T1-weighted black-blood imaging, a more specific ...
    Xenopus laevis Oocytes Preparation for in-Cell EPR Spectroscopy
    Authors:  Laura John and Malte Drescher, date: 04/05/2018, view: 819, Q&A: 0
    [Abstract] One of the most exciting perspectives for studying bio-macromolecules comes from the emerging field of in-cell spectroscopy, which enables to determine the structure and dynamics of bio-macromolecules in the cell. In-cell electron paramagnetic resonance (EPR) spectroscopy in combination with micro-injection of bio-macromolecules into Xenopus ...
    Modification and Application of a Commercial Whole-body Plethysmograph to Monitor Respiratory Abnormalities in Neonatal Mice
    Authors:  Yen-Ting Lai and Yi-Shuian Huang, date: 06/20/2017, view: 2496, Q&A: 0
    [Abstract] Proper breathing is essential for mammals to acquire oxygen after birth and requires coordinated actions among several tissues, including diaphragm, intercostal muscles, trachea, bronchi, lung and respiration-regulating neurons located in the medulla oblongata. Genetically modified mice that die early postnatally may have respiratory defects ...