Protocols in Current Issue
    Analysis of Monosaccharides from Arabidopsis Seed Mucilage and Whole Seeds Using HPAEC-PAD
    Authors:  Gillian H. Dean, Kresimir Sola, Faride Unda, Shawn D. Mansfield and George W. Haughn, date: 12/20/2019, view: 1710, Q&A: 0
    [Abstract] Arabidopsis seed coat epidermal cells deposit a significant quantity of mucilage, composed of the cell wall components pectin, hemicellulose, and cellulose, into the apoplast during development. When mature seeds are hydrated, mucilage extrudes to form a gelatinous capsule around the seed. Determining the monosaccharide composition of ...
    Whole-seed Immunolabeling of Arabidopsis Mucilage Polysaccharides
    Author:  Cătălin Voiniciuc, date: 06/05/2017, view: 4703, Q&A: 0
    [Abstract] In addition to synthesizing and secreting copious amounts of pectic polymers (Young et al., 2008), Arabidopsis thaliana seed coat epidermal cells produce small amounts of cellulose and hemicelluloses typical of secondary cell walls (Voiniciuc et al., 2015c). These components are intricately linked and are released as a ...
    Analysis of Monosaccharides in Total Mucilage Extractable from Arabidopsis Seeds
    Authors:  Cătălin Voiniciuc and Markus Günl, date: 05/05/2016, view: 7061, Q&A: 0
    [Abstract] The Arabidopsis thaliana seed coat epidermis produces copious amounts of mucilage polysaccharides (Haughn and Western, 2012). Characterization of mucilage mutants has identified novel genes required for cell wall biosynthesis and modification (North et al., 2014). The biochemical analysis of seed mucilage is essential to evaluate ...
    Quantification of the Mucilage Detachment from Arabidopsis Seeds
    Author:  Cătălin Voiniciuc, date: 05/05/2016, view: 5545, Q&A: 0
    [Abstract] The Arabidopsis thaliana seed coat produces large amounts of cell wall polysaccharides, which swell out of the epidermal cells upon hydration of the mature dry seeds. While most mucilage polymers immediately diffuse in the surrounding solution, the remaining fraction tightly adheres to the seed, forming a dense gel-like capsule (Macquet ...

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