Mammalia

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    Protocols in Current Issue
    A Simple and Fast Battery Test for Phenotypic Characterization of Mice
    [Abstract] Despite the great number of test batteries already known to assess the behavior of genetically modified and inbred strains of mice, only a few of them focus on basic neurological parameters. The purpose of the battery test proposed is to settle a specific methodology to characterize the phenotype of neurological disease models in mutant or ...
    Measurement of Dipeptidylpeptidase Activity in vitro and in vivo
    Authors:  Rosa Barreira da Silva, Molly A. Ingersoll and Matthew L. Albert, date: 03/20/2017, view: 5183, Q&A: 0
    [Abstract] Dipeptidylpeptidases (DPPs) are serine proteases, which cleave small proteins and peptides possessing a proline or an alanine in the second position of their N-terminus. Among the members of this family, dipeptidylpeptidase 4 (DPP4) is constitutively expressed in the extracellular space. DPP4 is found at the surface of many hematopoietic and ...
    Measurement of Transferrin- and Non-transferrin-bound Iron Uptake by Mouse Tissues
    Authors:  Supak Jenkitkasemwong, Chia-Yu Wang and Mitchell D. Knutson, date: 09/05/2016, view: 5633, Q&A: 0
    [Abstract] Iron in blood plasma is bound to its transport protein transferrin, which delivers iron to most tissues. In iron overload and certain pathological conditions, the carrying capacity of transferrin can become exceeded, giving rise to non-transferrin-bound iron, which is taken up preferentially by the liver, kidney, pancreas, and heart. The ...
    Measurement of 2-methylthio Modifications in Mitochondrial Transfer RNAs by Reverse-transcription Quantitative PCR
    Authors:  Fan-Yan Wei and Kazuhito Tomizawa, date: 01/05/2016, view: 5652, Q&A: 0
    [Abstract] 2-Methylthio-N6-isopentenyladenosine (ms2i6A) is an evolutionally conserved posttranscriptional modification found at position 37 of four mammalian mitochondrial tRNAs, mt-tRNASer(UCN), mt-tRNATrp, mt-tRNAPhe and mt-tRNATyr. The ms2 modification in ms2 ...



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