Mammalia

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    Protocols in Current Issue
    Isoelectric Focusing to Quantify Rhodopsin Phosphorylation in Mouse Retina
    Authors:  Sowmya Bekshe Lokappa, M. Carter Cornwall and Jeannie Chen, date: 07/20/2019, view: 1266, Q&A: 0
    [Abstract] Rhodopsin is a G-protein coupled receptor (GPCR) that mediates vision under dim light. Upon light exposure, rhodopsin is phosphorylated at multiple serine and threonine sites at its carboxyl-terminus by rhodopsin kinase (GRK1). This, in turn, reduces its ability to activate the visual G-protein transducin. Binding of light-activated, ...
    Assessment of Murine Retinal Function by Electroretinography
    Authors:  Gillie Benchorin, Melissa A. Calton, Marielle O. Beaulieu and Douglas Vollrath, date: 04/05/2017, view: 8081, Q&A: 0
    [Abstract] The electroretinogram (ERG) is a sensitive and noninvasive method for testing retinal function. In this protocol, we describe a method for performing ERGs in mice. Contact lenses on the mouse cornea measure the electrical response to a light stimulus of photoreceptors and downstream retinal cells, and the collected data are analyzed to evaluate ...
    Animal Models of Corneal Injury
    Authors:  Matilda F. Chan and Zena Werb, date: 07/05/2015, view: 6185, Q&A: 0
    [Abstract] The cornea is an excellent model system to use for the analysis of wound repair because of its accessibility, lack of vascularization, and simple anatomy. Corneal injuries may involve only the superficial epithelial layer or may penetrate deeper to involve both the epithelial and stromal layers. Here we describe two well-established in vivo ...



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