Protocols in Current Issue
    Cell Wall Biomass Preparation and Fourier Transform Mid-infrared (FTIR) Spectroscopy to Study Cell Wall Composition
    Authors:  Ricardo M. F. da Costa, Gordon G. Allison and Maurice Bosch, date: 06/05/2015, view: 6931, Q&A: 0
    [Abstract] Plant cell wall biomass is an abundant and renewable organic resource. Of the polymers it encloses, cellulose and hemicellulose are regarded as a raw material for the production of fuels and other products (Klemm et al., 2005; Slavov et al., 2013). Nonetheless, current usage of lignocellulosic biomass is still below its full ...
    Cyclohexane Diamine Tetraacetic Acid (CDTA) Extraction of Plant Cell Wall Pectin
    Authors:  Gerit Bethke and Jane Glazebrook, date: 12/20/2014, view: 8915, Q&A: 0
    [Abstract] The goal of this procedure is to extract pectin from plant cell walls. Pectins are galacturonic acid containing polymeric sugars that are important components of plant cell walls. Various procedures aimed at studying plant cell wall components require the extraction of pectin. Pectin is synthesized in the Golgi apparatus in a highly esterified ...
    In vitro Assay of the Glycosyltransferase Activity of a Heterologously Expressed Plant Protein
    Authors:  Carsten P Ade, Felix Bemm, James M J Dickson, Christian Walter and Philip J Harris, date: 11/05/2014, view: 8637, Q&A: 0
    [Abstract] Glycosyltransferases are carbohydrate active enzymes containing catalytic modules involved in catalysing the biosynthesis of glycosidic bonds in oligo- and polysaccharides and glycoconjugates. One of the most comprehensive collections of Carbohydrate Active enZYmes is the CAZy database ( ...
    Sandwich Enzyme-linked Immunosorbent Assay (ELISA) Analysis of Plant Cell Wall Glycan Connections
    Authors:  Valérie Cornuault and J. Paul Knox, date: 04/20/2014, view: 12990, Q&A: 0
    [Abstract] Sandwich ELISA is a highly sensitive method that can be used to determine if two epitopes are part of the same macromolecule or supramolecular complex. In the case of plant cell wall glycans, it can reveal the existence of inter-polymers linkages, leading to better understanding of overall cell wall architectures. This development of a ...

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