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    Protocols in Current Issue
    Pyrosequencing Approach for SNP Genotyping in Plants Using a M13 Biotinylated Primer
    Authors:  Cristina Silvar, Dragan Perovic, Anna M Casas, Ernesto Igartua and Frank Ordon, date: 05/20/2015, view: 6198, Q&A: 0
    [Abstract] Single Nucleotide Polymorphisms (SNPs), which constitute single base-pair variations in the DNA sequence, are the most abundant molecular markers in plant and animal genomes. They are becoming the markers of choice for genotyping in all fields of molecular biology, as they are easily prone to automation and high throughput, for example through ...
    Fluorescence-based CAPS Multiplex Genotyping on Capillary Electrophoresis Systems
    Authors:  Jelena Perovic, Cristina Silvar, Dragan Perovic, Nils Stein and Frank Ordon, date: 05/20/2015, view: 5768, Q&A: 0
    [Abstract] Recent advances in next-generation sequencing techniques allow the detection of a large number of SNPs and their use in a high throughput manner. However, Cleaved Amplified Polymorphic Sequences (CAPSs) still play a significant role as complement to other high throughput methods for SNP genotyping. Therefore, new methods focusing on the ...
    Modified Single-Cell Transient Gene Expression Assay in Barley Epidermal Cells
    Authors:  Shiwei Bai, Cheng Chang, Xinyun Han and Qian-Hua Shen, date: 05/05/2013, view: 6512, Q&A: 0
    [Abstract] Transient gene expression via biolistic particle delivery is a widely used technique for gene functional analysis in plants. In this protocol we describe a modified single-cell transient expression assay through transformation with a particle inflow gun of the model PDS-1000/He system (Bio-Rad). This assay was originally optimized for analyzing ...



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