Mammalia

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    Protocols in Current Issue
    In vivo Bioluminescence Imaging of Luciferase-labeled Cancer Cells
    [Abstract] Over the past decade, in vivo bioluminescent imaging has emerged as a non-invasive and sensitive tool for studying ongoing biological processes within living organisms (Contag et al., 1997; Contag et al., 1998). Based on the detection and quantitation of the photons produced by the oxidation of luciferin by luciferase ...
    Preparation of Candida albicans Biofilms Using an in vivo Rat Central Venous Catheter Model
    Authors:  Heather T. Taff, Karen Marchillo and David R. Andes, date: 07/20/2013, view: 6190, Q&A: 0
    [Abstract] In vivo biofilms grown on medical devices are necessary to understand the interactions of the fungal biofilm and the host environment in which it is most commonly found. This protocol describes a way to grow Candida albicans biofilms on the interior lumen of central venous catheters surgically implanted into rats, which mimics ...
    Iron - Prussian Blue Reaction - Mallory’s Method
    Author:  Hani Jouihan, date: 07/05/2012, view: 19406, Q&A: 0
    [Abstract] Purpose: To demonstrate ferric iron in tissue sections. Small amounts of iron are found normally in spleen and bone marrow. Excessive amounts are present in hemochromatosis, with deposits found in the liver and pancreas, hemosiderosis, with deposits in the liver, spleen, and lymph nodes.

    Principle: The reaction occurs with the treatment ...



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