Fungi

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    Protocols in Current Issue
    Visualizing Hypoxia in a Murine Model of Candida albicans Infection Using in vivo Biofluorencence
    Authors:  José Pedro Lopes and Constantin F. Urban, date: 08/05/2019, view: 291, Q&A: 0
    [Abstract] Candida albicans is a leading human fungal pathogen that uses several metabolic adaptations to escape immune cells and causes systemic disease. Here, we describe a protocol for measuring one of these adaptations, the ability to thrive in hypoxic niches. Hypoxia was generated after successful subdermal infection with C. albicans ...
    A Flow-assay for Farnesol Removal from Adherent Candida albicans Cultures
    Authors:  Melanie Polke and Ilse D. Jacobsen, date: 10/05/2017, view: 3420, Q&A: 0
    [Abstract] Here, we describe a protocol for a continuous flow system for C. albicans cultures growing adherent to a plastic surface. The protocol was adapted from a previous method established to simulate blood flow on endothelial cells (Wilson and Hube, 2010). The adapted protocol was used by us for the removal of molecules in C. albicans ...
    In vitro Analysis for Macrophage Binding and Pro-inflammatory Responses to Candida albicans
    Author:  Mohlopheni J. Marakalala, date: 05/05/2014, view: 5770, Q&A: 0
    [Abstract] Macrophage recognition of Candida albicans (C. albicans) is facilitated by pattern recognition receptors that interact with the fungal pathogen associated molecular patterns (PAMPs). Dectin-1 is the major macrophage receptor that is known to recognize fungal Beta-glucans leading to induction of various immune responses. This ...
    Preparation of Candida albicans Biofilms for Transmission Electron Microscopy
    Authors:  Heather T. Taff and David R. Andes, date: 07/20/2013, view: 7672, Q&A: 0
    [Abstract] Transmission Electron Microscopy is a form of microscopy that allows for imaging of distinct portions of an individual cell. For Candida albicans biofilms, it is often used to visualize the cell walls of fixed samples of yeast and hyphae. This protocol describes how to grow, harvest, and fix Candida albicans biofilms in ...
    Preparation of Mitochondria from Candida albicans
    Authors:  Victoria L Hewitt, Trevor Lithgow and Ana Traven, date: 02/20/2013, view: 7000, Q&A: 0
    [Abstract] Based on the methods of (Daum et al., 1982) and (Hewitt et al., 2012), we have established the use of Candida albicans as a new model system to study mitochondrial biogenesis. This dimorphic yeast provides an excellent system to investigate the coordination of mitochondrial biogenesis with other cellular networks ...



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