Protozoans

Categories

    Protocols in Current Issue
    Isolation of Latex Bead Phagosomes from Dictyostelium for in vitro Functional Assays
    Authors:  Ashwin D’Souza, Paulomi Sanghavi, Ashim Rai, Divya Pathak and Roop Mallik, date: 12/05/2016, view: 5698, Q&A: 0
    [Abstract] We describe a protocol to purify latex bead phagosomes (LBPs) from Dictyostelium cells. These can be later used for various in vitro functional assays. For instance, we use these LBPs to understand the microtubule motor-driven transport on in vitro polymerized microtubules. Phagosomes are allowed to mature for defined ...
    Dictyostelium Cultivation, Transfection, Microscopy and Fractionation
    Authors:  Jennifer Hirst, Robert R Kay and David Traynor, date: 06/05/2015, view: 9181, Q&A: 0
    [Abstract] The real time visualisation of fluorescently tagged proteins in live cells using ever more sophisticated microscopes has greatly increased our understanding of the dynamics of key proteins during fundamental physiological processes such as cell locomotion, chemotaxis, cell division and membrane trafficking. In addition the fractionation of cells ...



    We use cookies on this site to enhance your user experience. By using our website, you are agreeing to allow the storage of cookies on your computer.