Protocols in Current Issue
    Preparation of Mosquito Salivary Gland Extract and Intradermal Inoculation of Mice
    Authors:  Michael A. Schmid, Elizabeth Kauffman, Anne Payne, Eva Harris and Laura D. Kramer, date: 07/20/2017, view: 7062, Q&A: 2
    [Abstract] Mosquito-transmitted pathogens are among the leading causes of severe disease and death in humans. Components within the saliva of mosquito vectors facilitate blood feeding, modulate host responses, and allow efficient transmission of pathogens, such as Dengue, Zika, yellow fever, West Nile, Japanese encephalitis, and chikungunya viruses, as well ...
    Plasmodium Sporozoite Motility on Flat Substrates
    Authors:  Henriette L Prinz, Julia M Sattler and Friedrich Frischknecht, date: 07/20/2017, view: 3989, Q&A: 0
    [Abstract] Plasmodium sporozoites are the infectious, highly motile forms of the malaria parasite transmitted by Anopheles mosquitoes. Sporozoite motility can be assessed following the dissection of Anopheles salivary glands and isolation of sporozoites in vitro.
    Fluorescence in situ Hybridization to the Polytene Chromosomes of Anopheles Mosquitoes
    Authors:  Ai Xia, Ashley Peery, Maryam Kamali, Jiangtao Liang, Maria V. Sharakhova and Igor V. Sharakhov, date: 08/20/2013, view: 8283, Q&A: 0
    [Abstract] Fluorescence in situ hybridization (FISH) is a method that uses a fluorescently labeled DNA probe for mapping the position of a genetic element on chromosomes. A DNA probe is prepared by incorporating Cy-3 or Cy-5 labeled nucleotides into DNA by nick-translation or a random primed labeling method. This protocol was used to map genes ...

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