Fungi

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    Protocols in Current Issue
    Large-scale Phenotypic Profiling of Gene Deletion Mutants in Candida glabrata
    Authors:  Fabian Istel, Tobias Schwarzmüller, Michael Tscherner and Karl Kuchler, date: 07/20/2015, view: 6905, Q&A: 0
    [Abstract] Here, we describe a method enabling the phenotypic profiling of genome-scale deletion collections of fungal mutants to detect phenotypes for various stress conditions. These stress conditions include among many others antifungal drug susceptibility, temperature-induced and osmotic as well as heavy metal or oxidative stress. The protocol was ...
    Genetic Transformation of Candida glabrata by Heat Shock
    Authors:  Fabian Istel, Tobias Schwarzmüller, Michael Tscherner and Karl Kuchler, date: 07/20/2015, view: 5973, Q&A: 0
    [Abstract] Here, we report a method for the transformation of Candida glabrata using a heat shock method. The protocol can be used for transformations in single well or in 96-well scale. It has been employed as an alternative method to the electroporation protocol to construct a genome-scale gene deletion collection in the human fungal pathogen ...
    Genetic Transformation of Candida glabrata by Electroporation
    Authors:  Fabian Istel, Tobias Schwarzmüller, Michael Tscherner and Karl Kuchler, date: 07/20/2015, view: 5325, Q&A: 0
    [Abstract] Here, we report a method for the transformation by electroporation of the human fungal pathogen Candida glabrata (C. glabrata). The protocol can be used for transformations in single well or in 96-well microtiter plates. It has been extensively used to generate a genome-scale gene deletion library using the C. glabrata ...
    Catalase Activity Assay in Candida glabrata
    [Abstract] Commensal and pathogenic fungi are exposed to hydrogen peroxide (H2O2) produced by macrophages of the host. Pathogenic fungi counteract the harmful effects of H2O2 with the enzyme catalase (EC 1.11.1.6), which decomposes two molecules of H2O2 to two molecules of H2O and O ...
    Minimum Inhibitory Concentration (MIC) Assay for Antifungal Drugs
    Authors:  Jinglin L. Xie, Sheena D. Singh-Babak and Leah E. Cowen, date: 10/20/2012, view: 21498, Q&A: 0
    [Abstract] The Minimum Inhibitory Concentration (MIC) Assay is widely used to measure the susceptibility of yeasts to antifungal agents. In serial two-fold dilutions, the lowest concentration of antifungal drug that is sufficient to inhibit fungal growth is the MIC. Typically, 50% inhibitory (MIC50) or 80% inhibitory (MIC80) values are ...



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