Protocols in Current Issue
    Quantification of Bacterial Polyhydroxybutyrate Content by Flow Cytometry
    Authors:  Antonio Lagares (Jr.) and Claudio Valverde, date: 12/05/2017, view: 4038, Q&A: 0
    [Abstract] We describe here a detailed protocol for the quantification of the intracellular content of polyhydroxybutyrate (PHB) in a population of bacterial cells by flow cytometry, which is based on a consensus of several previously reported works.
    Quantification of Triphenyl-2H-tetrazoliumchloride Reduction Activity in Bacterial Cells
    Authors:  Roberto Defez, Anna Andreozzi and Carmen Bianco, date: 01/20/2017, view: 6351, Q&A: 0
    [Abstract] This protocol describes the use of the 2,3,5-triphenyl-2H-tetrazolium chloride (TTC) salt to evaluate the cell redox potential of rhizobia cells. The production of brightly colored and insoluble 1,3,5-Triphenyltetrazolium formazan arising from TTC reduction is irreversible and can be easily quantified using a spectrophotometer. This protocol ...
    Quantitative Evaluation of Competitive Nodulation among Different Sinorhizobium Strains
    Authors:  Chan Lan Chun, Matthew S. Nelson and Michael J. Sadowksy, date: 08/05/2015, view: 5245, Q&A: 0
    [Abstract] Legumes play a vital role in global food supply because they are uniquely capable of fixing atmospheric nitrogen (N) through symbioses with root and stem nodule bacteria, collectively called the rhizobia. These commonly include bacteria in the genera Rhizobium, Mesorhizobium, Sinorhizobium (Ensifer), and Bradyrhizobium ...
    Evaluation of Nodulation Speed by Sinorhizobium Strains
    Authors:  Matthew S. Nelson, Chan Lan Chun and Michael J. Sadowksy, date: 08/05/2015, view: 6120, Q&A: 0
    [Abstract] Rhizobia interact symbiotically with legumes to form root nodules, where by rhizobia fix atmospheric dinitrogen into ammonia in exchange for carbon produced via photosynthesis. The symbiotic interaction is agriculturally important by reducing the need for fertilizer containing nitrogen. The root and stem nodule bacteria commonly include bacteria ...

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