Plant Science

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    Protocols in Current Issue
    Determination of Recombinant Mannitol-1-phosphate Dehydrogenase Activity from Ectocarpus sp.
    Authors:  Agnès Groisillier and Thierry Tonon, date: 11/05/2016, view: 5484, Q&A: 0
    [Abstract] Brown algae belong to a phylogenetic lineage distantly related to green plants and animals, and are found predominantly, but not exclusively, in the intertidal zone, a harsh and frequently changing environment. Because of their unique evolutionary history and of their habitat, brown algae feature several peculiarities in their metabolism. One of ...
    Determination of Recombinant Mannitol-1-phosphatase Activity from Ectocarpus sp.
    Authors:  Agnès Groisillier and Thierry Tonon, date: 08/20/2016, view: 5826, Q&A: 0
    [Abstract] Brown algae belong to a phylogenetic lineage distantly related to green plants and animals, and are found predominantly, but not exclusively, in the intertidal zone, a harsh and frequently changing environment. Because of their unique evolutionary history and of their habitat, brown algae feature several peculiarities in their metabolism. One of ...
    Assay of the Carboxylase Activity of Rubisco from Chlamydomonas reinhardtii
    [Abstract] The performance of the carbon-fixing enzyme, ribulose 1, 5-bisphosphate carboxylase/oxygenase (EC 4.1.1.39, Rubisco), controls biomass accumulation in green plants, algae and most autotrophic bacteria. In particular, the carboxylase activity of Rubisco incorporates carbon from CO2 to ribulose 1, 5-bisphosphate (RuBP) producing two ...
    Pyruvate:ferredoxin Oxidoreductase (PFR1) Activity Assays Using Methyl Viologen as Artificial Electron Acceptor
    Author:  Jens Noth, date: 09/05/2013, view: 9737, Q&A: 0
    [Abstract] Here we describe the activity measurements of heterologous expressed pyruvate:ferredoxin oxidoreductase (Noth et al., 2013) (Noth et al.,2013) from Chlamydomonas reinhardtii. This enzyme catalyzes the reversible reaction (I) from pyruvate to acetyl CoA and CO2 generating low potential electrons which are in ...



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