Cancer Biology

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    Protocols in Current Issue
    Telomere Restriction Fragment (TRF) Analysis
    Authors:  Ilgen Mender and Jerry W. Shay, date: 11/20/2015, view: 13348, Q&A: 0
    [Abstract] While telomerase is expressed in ~90% of primary human tumors, most somatic tissue cells except transiently proliferating stem-like cells do not have detectable telomerase activity (Shay and Wright, 1996; Shay and Wright, 2001). Telomeres progressively shorten with each cell division in normal cells, including proliferating stem-like cells, due to ...
    Telomerase Repeated Amplification Protocol (TRAP)
    Authors:  Ilgen Mender and Jerry W. Shay, date: 11/20/2015, view: 12121, Q&A: 1
    [Abstract] Telomeres are found at the end of eukaryotic linear chromosomes, and proteins that bind to telomeres protect DNA from being recognized as double-strand breaks thus preventing end-to-end fusions (Griffith et al., 1999). However, due to the end replication problem and other factors such as oxidative damage, the limited life span of cultured ...
    Telomere Dysfunction Induced Foci (TIF) Analysis
    Authors:  Ilgen Mender and Jerry W. Shay, date: 11/20/2015, view: 6703, Q&A: 0
    [Abstract] Telomerase maintains telomeric DNA in eukaryotes during early developments, ~90% of cancer cells and some proliferative stem like cells. Telomeric repeats at the end of chromosomes are associated with the shelterin complex. This complex consists of TRF1, TRF2, Rap1, TIN2, TPP1, POT1 which protect DNA from being recognized as DNA double-stranded ...



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